三种重组氨基糖苷修饰酶对维替米星的修饰敏感性。
Susceptibility of vertilmicin to modifications by three types of recombinant aminoglycoside-modifying enzymes.
机构信息
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
出版信息
Antimicrob Agents Chemother. 2011 Aug;55(8):3950-3. doi: 10.1128/AAC.00300-11. Epub 2011 Jun 6.
Abstract
The susceptibilities of vertilmicin and seven reference aminoglycosides to modifications by six recombinant aminoglycoside-modifying enzymes, AAC(6')-Ie, APH(2'')-Ia, AAC(6')-Ie-APH(2'')-Ia, ANT(2'')-Ia, AAC(6')-Ib, and AAC(6')-Ib-cr, were studied by coupled spectrophotometric assays in microtiter plates. In comparison to other aminoglycosides, the susceptibility of vertilmicin was 45.8- to 250.0-fold lower for AAC(6')-Ie acetylation, 39.2- to 116.7-fold lower for AAC(6')-Ie-APH(2'')-Ia acetylation, and 1.8- to 7.5-fold lower for ANT(2'')-Ia adenylation (except that shown by amikacin) while relatively comparable for AAC(6')-Ib acetylation, AAC(6')-Ib-cr acetylation, APH(2'')-Ia phosphorylation, and AAC(6')-Ie-APH(2'')-Ia phosphorylation.
摘要
采用微量板偶联分光光度法研究了委内霉素和 7 种参考氨基糖苷类药物对 6 种重组氨基糖苷类修饰酶（AAC(6')-Ie、APH(2'')-Ia、AAC(6')-Ie-APH(2'')-Ia、ANT(2'')-Ia、AAC(6')-Ib 和 AAC(6')-Ib-cr）的修饰敏感性。与其他氨基糖苷类药物相比，AAC(6')-Ie 乙酰化使委内霉素的敏感性降低了 45.8-250.0 倍，AAC(6')-Ie-APH(2'')-Ia 乙酰化降低了 39.2-116.7 倍，而 ANT(2'')-Ia 腺苷酰化降低了 1.8-7.5 倍（阿米卡星除外），而 AAC(6')-Ib 乙酰化、AAC(6')-Ib-cr 乙酰化、APH(2'')-Ia 磷酸化和 AAC(6')-Ie-APH(2'')-Ia 磷酸化的相对比较则相当。
相似文献
1
Susceptibility of vertilmicin to modifications by three types of recombinant aminoglycoside-modifying enzymes.三种重组氨基糖苷修饰酶对维替米星的修饰敏感性。
Antimicrob Agents Chemother. 2011 Aug;55(8):3950-3. doi: 10.1128/AAC.00300-11. Epub 2011 Jun 6.
2
In vitro antibacterial activity of vertilmicin and its susceptibility to modifications by the recombinant AAC6'-APH2'' enzyme.韦替米星的体外抗菌活性及其对重组 AAC6'-APH2'' 酶修饰的敏感性。
Antimicrob Agents Chemother. 2008 Nov;52(11):3875-82. doi: 10.1128/AAC.01400-07. Epub 2008 Aug 18.
3
Molecular identification of aminoglycoside-modifying enzymes in clinical isolates of Escherichia coli resistant to amoxicillin/clavulanic acid isolated in Spain.在西班牙分离的耐阿莫西林/克拉维酸的临床大肠杆菌分离株中氨基糖苷类修饰酶的分子鉴定。
Int J Antimicrob Agents. 2015 Aug;46(2):157-63. doi: 10.1016/j.ijantimicag.2015.03.008. Epub 2015 Apr 29.
4
Genetic diversity of enterococci harboring the high-level gentamicin resistance gene aac(6')-Ie-aph(2'')-Ia or aph(2'')-Ie in a Japanese hospital.日本一家医院中携带高水平庆大霉素耐药基因aac(6')-Ie-aph(2'')-Ia或aph(2'')-Ie的肠球菌的遗传多样性
Microb Drug Resist. 2009 Sep;15(3):185-94. doi: 10.1089/mdr.2009.0917.
5
Conjugal transfer of aac(6')Ie-aph(2″)Ia gene from native species and mechanism of regulation and cross resistance in Enterococcus faecalis MCC3063 by real time-PCR.粪肠球菌MCC3063中aac(6')Ie-aph(2″)Ia基因的种内接合转移及调控与交叉耐药机制的实时荧光定量PCR分析
Microb Pathog. 2017 Sep;110:546-553. doi: 10.1016/j.micpath.2017.07.049. Epub 2017 Jul 31.
6
Genetic basis of enzymatic resistance of E. coli to aminoglycosides.大肠杆菌对氨基糖苷类抗生素产生酶抗性的遗传基础。
Adv Med Sci. 2018 Mar;63(1):9-13. doi: 10.1016/j.advms.2017.05.004. Epub 2017 Jul 29.
7
[Molecular identification of aminoglycoside-modifying enzymes among strains of Enterococcus spp. isolated in hospitals of the VIII Region of Chile].[智利第八大区医院分离的肠球菌属菌株中氨基糖苷类修饰酶的分子鉴定]
Rev Med Chil. 2007 May;135(5):566-72. doi: 10.4067/s0034-98872007000500003. Epub 2007 Jul 9.
8
Prodigious substrate specificity of AAC(6')-APH(2"), an aminoglycoside antibiotic resistance determinant in enterococci and staphylococci.AAC(6')-APH(2")的巨大底物特异性，这是一种在肠球菌和葡萄球菌中的氨基糖苷类抗生素抗性决定因素。
Chem Biol. 1999 Feb;6(2):99-110. doi: 10.1016/S1074-5521(99)80006-4.
9
Revisiting the nucleotide and aminoglycoside substrate specificity of the bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia enzyme.重新研究双功能氨基糖苷乙酰转移酶（6'）-Ie/氨基糖苷磷酸转移酶（2'）-Ia 酶的核苷酸和氨基糖苷类底物特异性。
J Biol Chem. 2012 Dec 21;287(52):43262-9. doi: 10.1074/jbc.M112.416453. Epub 2012 Oct 31.
10
Novel aminoglycoside 2''-phosphotransferase identified in a gram-negative pathogen.在一种革兰氏阴性病原体中发现的新型氨基糖苷 2''-磷酸转移酶。
Antimicrob Agents Chemother. 2013 Jan;57(1):452-7. doi: 10.1128/AAC.02049-12. Epub 2012 Nov 5.
引用本文的文献
1
Identification of a novel aminoglycoside nucleotidyltransferase gene in Morganella morganii from farm sewage.从农场污水摩根氏摩根菌中鉴定出一种新型氨基糖苷核苷酸转移酶基因。
BMC Microbiol. 2025 Mar 21;25(1):161. doi: 10.1186/s12866-025-03844-5.
2
ANT(9)-Ic, a Novel Chromosomally Encoded Aminoglycoside Nucleotidyltransferase from Brucella intermedia.中间布鲁氏菌中新型染色体编码氨基糖苷核苷转移酶 ANT(9)-Ic。
Microbiol Spectr. 2023 Jun 15;11(3):e0062023. doi: 10.1128/spectrum.00620-23. Epub 2023 Apr 11.
3
Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing, China.中国北京鲍曼不动杆菌高水平氨基糖苷类耐药的遗传基础
Acta Pharm Sin B. 2014 Aug;4(4):295-300. doi: 10.1016/j.apsb.2014.06.004. Epub 2014 Jul 15.
4
Small-angle X-ray scattering analysis of the bifunctional antibiotic resistance enzyme aminoglycoside (6') acetyltransferase-ie/aminoglycoside (2'') phosphotransferase-ia reveals a rigid solution structure.小角 X 射线散射分析双功能抗生素耐药酶氨基糖苷（6'）乙酰基转移酶-ie/氨基糖苷（2'）磷酸转移酶-ia 揭示了其刚性的溶液结构。
Antimicrob Agents Chemother. 2012 Apr;56(4):1899-906. doi: 10.1128/AAC.06378-11. Epub 2012 Jan 30.
本文引用的文献
1
Aminoglycoside modifying enzymes.氨基糖苷修饰酶。
Drug Resist Updat. 2010 Dec;13(6):151-71. doi: 10.1016/j.drup.2010.08.003. Epub 2010 Sep 15.
2
GES-11, a novel integron-associated GES variant in Acinetobacter baumannii.GES-11，鲍曼不动杆菌中一种新型的与整合子相关的GES变体。
Antimicrob Agents Chemother. 2009 Aug;53(8):3579-81. doi: 10.1128/AAC.00072-09. Epub 2009 May 18.
3
Complete nucleotide sequence of pKP96, a 67 850 bp multiresistance plasmid encoding qnrA1, aac(6')-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae.pKP96的完整核苷酸序列，这是一个来自肺炎克雷伯菌的67850碱基对的多重耐药质粒，编码qnrA1、aac(6')-Ib-cr和blaCTX-M-24。
J Antimicrob Chemother. 2008 Dec;62(6):1252-6. doi: 10.1093/jac/dkn397. Epub 2008 Sep 23.
4
In vitro antibacterial activity of vertilmicin and its susceptibility to modifications by the recombinant AAC6'-APH2'' enzyme.韦替米星的体外抗菌活性及其对重组 AAC6'-APH2'' 酶修饰的敏感性。
Antimicrob Agents Chemother. 2008 Nov;52(11):3875-82. doi: 10.1128/AAC.01400-07. Epub 2008 Aug 18.
5
Mechanistic and structural analysis of aminoglycoside N-acetyltransferase AAC(6')-Ib and its bifunctional, fluoroquinolone-active AAC(6')-Ib-cr variant.氨基糖苷N-乙酰基转移酶AAC(6')-Ib及其双功能、具有氟喹诺酮活性的AAC(6')-Ib-cr变体的机制和结构分析。
Biochemistry. 2008 Sep 16;47(37):9825-35. doi: 10.1021/bi800664x. Epub 2008 Aug 19.
6
16S ribosomal RNA methylation: emerging resistance mechanism against aminoglycosides.16S核糖体RNA甲基化：针对氨基糖苷类抗生素新出现的耐药机制
Clin Infect Dis. 2007 Jul 1;45(1):88-94. doi: 10.1086/518605. Epub 2007 May 21.
7
Mechanistic characterization of the bifunctional aminoglycoside-modifying enzyme AAC(3)-Ib/AAC(6')-Ib' from Pseudomonas aeruginosa.铜绿假单胞菌双功能氨基糖苷修饰酶AAC(3)-Ib/AAC(6')-Ib'的机制表征
Biochemistry. 2007 May 1;46(17):5270-82. doi: 10.1021/bi700111z. Epub 2007 Apr 7.
8
Genetic analysis of a multiresistant strain of Pseudomonas aeruginosa producing PER-1 beta-lactamase.一株产PER-1β-内酰胺酶的多重耐药铜绿假单胞菌的基因分析
Clin Microbiol Infect. 2006 Mar;12(3):270-8. doi: 10.1111/j.1469-0691.2005.01333.x.
9
Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase.氟喹诺酮修饰酶：一种常见氨基糖苷类乙酰转移酶的新适应性变体
Nat Med. 2006 Jan;12(1):83-8. doi: 10.1038/nm1347. Epub 2005 Dec 20.
10
Integron carrying a novel metallo-beta-lactamase gene, blaIMP-16, and a fused form of aminoglycoside-resistant gene aac(6')-30/aac(6')-Ib': report from the SENTRY Antimicrobial Surveillance Program.携带新型金属β-内酰胺酶基因blaIMP-16以及氨基糖苷类耐药基因aac(6')-30/aac(6')-Ib'融合形式的整合子：哨兵抗菌监测计划报告
Antimicrob Agents Chemother. 2004 Dec;48(12):4693-702. doi: 10.1128/AAC.48.12.4693-4702.2004.
Zotero 插件