白细胞介素 28B rs12979860 C/T 多态性对慢性丙型肝炎组织学结果的作用：与性别和病毒基因型的关系。
Role of interleukin 28B rs12979860 C/T polymorphism on the histological outcome of chronic hepatitis C: relationship with gender and viral genotype.
机构信息
Laboratory Medicine, University of Udine, Udine, Italy.
出版信息
J Clin Immunol. 2011 Oct;31(5):891-9. doi: 10.1007/s10875-011-9547-1. Epub 2011 Jun 7.
BACKGROUND
This study aimed to determine whether the single-nucleotide polymorphism (rs12979860 C/T) of the interleukin 28B (IL-28B) gene, which is associated with hepatitis C virus (HCV) clearance, is also associated with fibrosis in chronic HCV infection.
METHODS
An RFLP-PCR technique was used to genotype 629 HCV-positive patients (200 with cirrhosis) and 428 healthy control subjects.
RESULTS
The genotype frequencies in the controls and chronic hepatitis C patients were as follows: C/C 47.0% vs. 32.6%, C/T 41.8% vs. 52.8% and T/T 11.2% vs. 14.6% (p < 0.0001). The C allele frequency was higher in HCV-2- (0.635) and 3- (0.692) infected patients in comparison to those infected with HCV-1 (0.550) or 4-5 (0.600) (p < 0.001). Infected T/T homozygotes had a mean staging score higher than other patients (3.50 vs. 3.04, p < 0.05).
CONCLUSIONS
IL-28B rs12979860 C/T polymorphism is associated with a greater likelihood of HCV persistence, particularly in HCV genotypes 1 and 4. The T allele affects the severity of liver fibrosis.
背景
本研究旨在确定与丙型肝炎病毒 (HCV) 清除相关的白细胞介素 28B (IL-28B) 基因单核苷酸多态性 (rs12979860 C/T) 是否也与慢性 HCV 感染中的纤维化有关。
方法
使用 RFLP-PCR 技术对 629 名 HCV 阳性患者（200 名肝硬化患者）和 428 名健康对照进行基因分型。
结果
对照组和慢性丙型肝炎患者的基因型频率如下：C/C 47.0% vs. 32.6%，C/T 41.8% vs. 52.8%和 T/T 11.2% vs. 14.6%（p<0.0001）。与 HCV-1（0.550）或 HCV-4-5（0.600）感染患者相比，HCV-2-（0.635）和 HCV-3-（0.692）感染患者的 C 等位基因频率更高（p<0.001）。感染 T/T 纯合子的患者分期评分高于其他患者（3.50 与 3.04，p<0.05）。
结论
IL-28B rs12979860 C/T 多态性与 HCV 持续存在的可能性更大相关，特别是在 HCV 基因型 1 和 4 中。T 等位基因影响肝纤维化的严重程度。
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