Galal Al-Shazly Gaber Mohamed, Dawood Reham M, Awady Mostafa K El, El-Dessouky Yasser Mohamed Mohamed, Mahmoud Mohamed Mahmoud Abdel-Halim, Alla Mohamed Darwish Ahmed Abd
Horus Specialized Hospital, General Authority of Health Care, Waburat Armant, Egypt.
Department of Microbial Biotechnology, National Research Center, Cairo, Egypt.
J Genet Eng Biotechnol. 2023 Aug 30;21(1):89. doi: 10.1186/s43141-023-00544-3.
Predictors of chronic HCV response to oral antiviral therapy (OAT) are related to host genetic variations. Single nucleotide polymorphisms (SNP) and alleles variations of host genes in association with hepatic fibro-cirrhotic changes have a distinct role in OAT outcomes. The current research evaluated the association of Cirrhosis-Risk-Scores (CRS) values, based on the correlation of seven genes signature-SNPs, with sonographic liver parenchymal changes in determining OAT outcomes.
All study subjects (n = 54) were recruited three months after completing OAT and classified into three groups. Group I (n = 21) had negative HCV PCR, group II (n = 17) showed positive solitary intra-PBMCs HCV infection, and group III(n = 16) was serum HCV RNA PCR-positive. All study-population were subjected to examination by hepatic-ultrasound (US), FIB-4-scoring, and screening for 7 gene-signature that addressed CRS values as low, intermediate, and high depending on gene SNPs identification.
Group I showed a significant association with low CRS values compared to other groups (P < 0.001). Solitary intra- PBMCs HCV infection in group II was significantly combined with intermediate CRS values in comparison to groups I and III (P < 0.001). The high CRS values were significantly found in group III when compared to groups I and II (P < 0.01). On US imaging, low CRS values were common in normally appeared hepatic parenchyma (P < 0.001) and high CRS values were frequent in coarse-liver (P < 0.001), while bright-liver-tissues appearance was mainly detected in the intermediate CRS category (P = 0.09). On FIB-4 scoring, high CRS value were associated with hepatic fibro-cirrhosis compared to intermediate (P < 0.001) and low (P = 0.08) CRS-categories.
The current study concluded the association of (a) high CRS values with coarse liver in viral-RNA serologic relapse, (b) low CRS values with normal liver tissues in sustained virologic response (SVR), (c) intermediate CRS values with bright liver in solitary PBMCs relapse.
慢性丙型肝炎病毒（HCV）对口服抗病毒治疗（OAT）反应的预测因子与宿主基因变异有关。宿主基因的单核苷酸多态性（SNP）和等位基因变异与肝纤维化 - 肝硬化变化相关，在OAT治疗结果中具有独特作用。本研究基于七个基因特征性SNP的相关性，评估肝硬化风险评分（CRS）值与肝脏实质超声变化在确定OAT治疗结果中的关联。
所有研究对象（n = 54）在完成OAT三个月后入组，并分为三组。第一组（n = 21）HCV PCR检测为阴性，第二组（n = 17）显示单个外周血单核细胞内HCV感染阳性，第三组（n = 16）血清HCV RNA PCR检测为阳性。所有研究人群均接受肝脏超声（US）检查、FIB - 4评分，并筛查7个基因特征，根据基因SNP鉴定将CRS值分为低、中、高。
与其他组相比，第一组与低CRS值显著相关（P < 0.001）。与第一组和第三组相比，第二组单个外周血单核细胞内HCV感染与中等CRS值显著相关（P < 0.001）。与第一组和第二组相比，第三组中显著发现高CRS值（P < 0.01）。在超声成像中，正常肝脏实质中低CRS值常见（P < 0.001），粗肝中高CRS值常见（P < 0.001），而明亮肝脏组织外观主要在中等CRS类别中检测到（P = 0.09）。在FIB - 4评分中，与中等（P < 0.001）和低（P = 0.08）CRS类别相比，高CRS值与肝纤维化 - 肝硬化相关。
本研究得出以下关联：（a）高CRS值与病毒RNA血清学复发时的粗肝相关；（b）低CRS值与持续病毒学应答（SVR）时的正常肝组织相关；（c）中等CRS值与单个外周血单核细胞复发时的明亮肝脏相关。
