Dämmrich J, Müller-Hermelink H K, Mattner A, Buchwald J, Ziffer S
Pathologiches institut der universität Würzburg, luitpolddrankenhaus,Bundesrepublik Deutschland.
Cancer. 1990 May 1;65(9):1942-54. doi: 10.1002/1097-0142(19900501)65:9<1942::aid-cncr2820650912>3.0.co;2-6.
In 70 human carcinomas of the lung the expression of histocompatibility antigens (HLA) was investigated by using monoclonal antibodies in frozen sections. The immunmoreactive tumor volume was determined morphometrically. The tumor types showed a different pattern of HLA expressions. In squamous cell carcinomas (SQC) and small cell carcinomas (SCC) the HLA-A,B,C expression varied in a great range from 0% to 100% of positive tumor volume. For the most part, HLA-DR was not demonstrable. In adenocarcinomas (AC) and large cell carcinomas two different populations were observed. One showed a pattern of HLA expression as seen in SQC. Electron microscopically these AC (type 1) (AC/1) were characterized by intracytoplasmic mucin granules indicating a phenotypical relationship to goblet cells. The other--nearly 50% of adenocarcinomas and one third of large cell carcinomas--expressed both HLA antigens homogeneously in the total tumor volume. Electron microscopically the characteristics of type II pneumocytes and Clara cells were seen in tumor cells of these AC (type II) (AC/II), consisting in lamellar bodies and apically located electron-dense granules. In SQC and AC/I a significant correlation was found between grades of differentiation and mitotic activity on the one side, and expression of HLA-A,B,C on the otrher. The loss of HLA-A,B,C expression seemed to be an indication of a lower grade of differentiation; the smalles expression of HLA-A,B,C occurred in SCC.
采用单克隆抗体对70例人肺癌冷冻切片组织相容性抗原（HLA）的表达进行了研究。通过形态计量学方法测定免疫活性肿瘤体积。肿瘤类型显示出不同的HLA表达模式。在鳞状细胞癌（SQC）和小细胞癌（SCC）中，HLA - A、B、C的表达在阳性肿瘤体积的0%至100%范围内有很大差异。在大多数情况下，无法检测到HLA - DR。在腺癌（AC）和大细胞癌中观察到两种不同的细胞群。一种表现出与SQC中所见的HLA表达模式。电子显微镜下，这些AC（1型）（AC/1）的特征是胞浆内有粘蛋白颗粒，表明与杯状细胞有表型关系。另一种——近50%的腺癌和三分之一的大细胞癌——在整个肿瘤体积中均匀表达两种HLA抗原。电子显微镜下，在这些AC（II型）（AC/II）的肿瘤细胞中可见II型肺泡上皮细胞和克拉拉细胞的特征，包括板层小体和顶端定位的电子致密颗粒。在SQC和AC/I中，一方面分化程度和有丝分裂活性与另一方面HLA - A、B、C的表达之间存在显著相关性。HLA - A、B、C表达的缺失似乎表明分化程度较低；SCC中HLA - A、B、C的表达最低。
