Dumas D P, Wild J R, Raushel F M
Department of Chemistry, Texas A & M University, College Station 77843.
Experientia. 1990 Jul 15;46(7):729-31. doi: 10.1007/BF01939948.
The gene encoding for the phosphotriesterase (opd) from Pseudomonas diminuta has been subcloned into a baculovirus expression system. Functional enzyme is produced when the recombinant baculovirus is used to infect either cultured Spodoptera frugiperda sf9 cells or the larval stage of the fall armyworm. The LD50 for paraoxon toxicity was found to increase 280-fold in the larvae after infection with the recombinant baculovirus and expression of the functional phosphotriesterase.
