Shull G E
Department of Molecular Genetics, University of Cincinnati College of Medicine, Ohio 45267-0524.
J Biol Chem. 1990 Jul 25;265(21):12123-6.
A cDNA encoding the beta-subunit of the rat gastric H,K-ATPase has been identified using oligonucleotide probes based on the amino acid sequences of two peptides from the pig H,K-ATPase beta-subunit (Hall, K., Perez, G., Anderson, D., Gutierrez, C., Munson, K., Hersey, S. J., Kaplan, J. H., and Sachs, G. (1990) Biochemistry 29, 701-706). The nucleotide sequence of the 1.3-kilobase cDNA has been determined and the primary structure of the protein deduced. The protein consists of 294 amino acids and has an Mr of 33,625. The amino acid sequence of the H,K-ATPase beta-subunit is similar to those of the beta 1 (29% identity) and beta 2 (37% identity) subunits of the Na,K-ATPase. Based on the hydropathy profile it seems to have the same transmembrane organization as the Na,K-ATPase beta-subunit, with a single membrane-spanning domain near the amino terminus. Seven potential N-linked glycosylation sites are located in the putative extracellular regions of the protein. Northern blot analyses of poly(A)+ RNAs from 13 tissues demonstrate that the H,K-ATPase beta-subunit mRNA is expressed at high level in stomach and is not expressed in any of the other tissues.
利用基于猪H，K - ATP酶β亚基两条肽段氨基酸序列的寡核苷酸探针，已鉴定出编码大鼠胃H，K - ATP酶β亚基的cDNA（霍尔，K.，佩雷斯，G.，安德森，D.，古铁雷斯，C.，芒森，K.，赫西，S.J.，卡普兰，J.H.，以及萨克斯，G.（1990年）《生物化学》29卷，701 - 706页）。已确定了1.3千碱基cDNA的核苷酸序列，并推导了该蛋白质的一级结构。该蛋白质由294个氨基酸组成，分子量为33,625。H，K - ATP酶β亚基的氨基酸序列与钠钾ATP酶的β1亚基（29%的同一性）和β2亚基（37%的同一性）相似。根据亲水性图谱，它似乎与钠钾ATP酶β亚基具有相同的跨膜结构，在氨基末端附近有一个单一的跨膜结构域。七个潜在的N - 糖基化位点位于该蛋白质假定的细胞外区域。对来自13种组织的聚腺苷酸加尾RNA进行的Northern印迹分析表明，H，K - ATP酶β亚基mRNA在胃中高水平表达，而在其他任何组织中均不表达。
