Maeda M, Ishizaki J, Futai M
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
Biochem Biophys Res Commun. 1988 Nov 30;157(1):203-9. doi: 10.1016/s0006-291x(88)80033-0.
Complementary DNA to pig gastric mRNA encoding (H+ + K+)-ATPase was cloned, and its amino acid sequence was deduced from the nucleotide sequence. The enzyme contained 1034 amino acid residues (Mr. 114,285) including the initiation methionine. The sequence of pig (H+ + K+)-ATPase was highly homologous with that of the corresponding enzyme from rat, but had high degree of synonymous codon changes. Potential sites of phosphorylation by cAMP-dependent protein kinase and N-linked glycosylation sites were identified. The amino terminal region contained a lysine-rich sequence similar to that of the alpha subunit of (Na+ + K+)-ATPase, although a cluster of glycine residues was inserted into the sequence of the (H+ + K+)-ATPase. As the pig enzyme is advantageous for biochemical studies, the information of the primary structure is useful for further detailed studies.
克隆了编码猪胃（H⁺+K⁺）-ATP酶的互补DNA，并从核苷酸序列推导其氨基酸序列。该酶包含1034个氨基酸残基（分子量114285），包括起始甲硫氨酸。猪（H⁺+K⁺）-ATP酶的序列与大鼠相应酶的序列高度同源，但同义密码子变化程度较高。确定了依赖cAMP的蛋白激酶磷酸化的潜在位点和N-连接糖基化位点。氨基末端区域包含一个富含赖氨酸的序列，类似于（Na⁺+K⁺）-ATP酶的α亚基，尽管在（H⁺+K⁺）-ATP酶的序列中插入了一组甘氨酸残基。由于猪酶有利于生化研究，一级结构信息对进一步的详细研究很有用。
