Institute for Materials Research, Tohoku University, 2-1-1, Katahira, Sendai 980-8577, Japan.
Langmuir. 2011 Jul 5;27(13):8333-8. doi: 10.1021/la2010985. Epub 2011 Jun 9.
The effect of an external ac electric field on the nucleation rate of hen egg white lysozyme crystals increased with an increase in the concentration of the precipitant used, which enabled the design of an electric double layer (EDL) formed at the inner surface of the drop in the oil. This is attributed to the thickness of the EDL controlled by the ionic strength of the precipitant used. Control of the EDL formed at the interface between the two phases is important to establishing this novel technique for the crystallization of proteins under the application of an external ac electric field.
外加交流电场对蛋清溶菌酶晶体成核速率的影响随沉淀剂浓度的增加而增加，这使得可以设计在油滴内部表面形成的双电层（EDL）。这归因于由所用沉淀剂的离子强度控制的 EDL 的厚度。控制两相之间形成的 EDL 对于在外加交流电场下建立蛋白质结晶的这种新技术很重要。
