School Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 116016, China.
J Chromatogr A. 2011 Jul 22;1218(29):4606-11. doi: 10.1016/j.chroma.2011.05.048. Epub 2011 May 20.
Adsorption on polyamide resin was investigated as a means of separating lithospermic acid B (LAB) from a crude extract of the roots of the traditional Chinese medicine Salvia miltiorrhiza Bunge ("Danshen"). Variables affecting adsorption capacity (solution pH, contact time on resin, initial LAB concentration) were studied. Adsorption was strongly dependent upon the initial concentration of LAB and pH. In all conditions, the polyamide resin gave optimal adsorption of LAB at an initial concentration of 2.66 mg/mL and pH <3.0. The adsorption isotherm correlated well with the Langmuir-type adsorption isotherm. Maximal adsorption capacity was calculated to be 380 mg/g at pH 2.0 and 25°C. LAB purity of 85.30% could be obtained by polyamide resin adsorption followed by elution with 70% ethanol solution, and the recovery was 87.1%. After preparative HPLC, the maximum HPLC purity obtained was 99.28% with a recovery of 75.2%. This method provides an efficient and low-cost method for LAB purification for industrial applications.
以聚酰胺树脂为吸附剂，从丹参根的粗提物中分离紫草酸 B（LAB）。考察了影响吸附容量的变量（溶液 pH 值、树脂接触时间、初始 LAB 浓度）。吸附强烈依赖于初始 LAB 浓度和 pH 值。在所有条件下，聚酰胺树脂在初始浓度为 2.66mg/mL 和 pH 值<3.0 时对 LAB 具有最佳的吸附效果。吸附等温线与 Langmuir 型吸附等温线很好地相关。在 pH 值为 2.0 和 25°C 时，最大吸附容量计算为 380mg/g。用 70%乙醇溶液洗脱，经聚酰胺树脂吸附后，LAB 的纯度可达 85.30%，回收率为 87.1%。经制备型 HPLC 后，最大 HPLC 纯度为 99.28%，回收率为 75.2%。该方法为 LAB 的工业应用提供了一种高效、低成本的纯化方法。
