The State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, USA.
Comp Biochem Physiol Part D Genomics Proteomics. 2011 Sep;6(3):264-70. doi: 10.1016/j.cbd.2011.05.003. Epub 2011 May 24.
Roxarsone is a commonly used additive in chicken (Gallus gallus) industry. However, little is known on the intrinsic molecular mechanism via which the growth performance of birds improves. This study was therefore performed to investigate the expression profiles of genes induced by roxarsone. Fifty-six broiler chickens were divided into two groups, namely treated and untreated with roxarsone. The treated group was provided a diet of 45.4mg/kg roxarsone medication and the other group acted as control. Data analysis showed that roxarsone consistently and significantly (P<0.05) increased chicken growth performance. In addition to this a significant (P<0.05) increase of arsenic residue in liver has been seen. Microarray expression analysis of 8935 genes in liver showed that 22 genes (10 up- and 12 down-regulated) had altered expression throughout the experimental periods. Two novel genes (GenBank accession no. GU724343 and GU724344) were cloned through rapid amplification of cDNA ends (RACE). Gene GU724343 was predicted to encode an unidentified protein and the second gene GU724344 was presumed to encode a new member of immunoglobulin-like receptor (CHIR) family. Our results suggested for the first time that the role of roxarsone could be mainly to modify the expression levels of cell growth, immunity/defense and energy metabolism associated genes, as a result promoting animal growth. Further research on these genes should help to increase the knowledge of improving animal productivity safely and effectively.
罗硝唑是一种常用于家禽（鸡）行业的添加剂。然而，关于其改善禽类生长性能的内在分子机制知之甚少。因此，本研究旨在研究罗硝唑诱导的基因表达谱。将 56 只肉鸡分为两组，分别用罗硝唑处理和未处理。处理组提供含有 45.4mg/kg 罗硝唑药物的饮食，另一组作为对照。数据分析表明，罗硝唑持续且显著（P<0.05）提高了鸡的生长性能。此外，还观察到肝脏中砷残留量显著增加（P<0.05）。肝脏中 8935 个基因的微阵列表达分析显示，有 22 个基因（10 个上调和 12 个下调）在整个实验期间表达发生改变。通过快速扩增 cDNA 末端（RACE）克隆了两个新基因（GenBank 登录号 GU724343 和 GU724344）。基因 GU724343 预测编码一种未知蛋白，第二个基因 GU724344 被推测编码免疫球蛋白样受体（CHIR）家族的新成员。我们的研究结果首次表明，罗硝唑的作用主要可能是调节与细胞生长、免疫/防御和能量代谢相关的基因的表达水平，从而促进动物生长。对这些基因的进一步研究应该有助于增加安全有效地提高动物生产力的知识。
