Office of Regulatory Science, Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, College Park, Maryland 20740, United States.
J Agric Food Chem. 2012 Sep 19;60(37):9394-404. doi: 10.1021/jf302366a. Epub 2012 Sep 5.
Roxarsone, (4-hydroxy-3-nitrophenyl)arsonic acid, is an arsenic-containing compound that has been approved as a feed additive for poultry and swine since the 1940s; however, little information is available regarding residual arsenic species present in edible tissues. We developed a novel method for the extraction and quantification of arsenic species in chicken liver. A strongly basic solution solubilized the liver, and ultrafiltration removed macromolecules and particulate material. Ion chromatography separated the species [arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, (4-hydroxy-3-aminophenyl)arsonic acid, (4-hydroxy-3-acetaminophenyl)arsonic acid, and roxarsone] in the extracts, which were then detected by inductively coupled plasma-mass spectrometry. The extraction oxidized most arsenite to arsenate. For fortification concentrations at 2 μg kg(-1) and above, recoveries ranged from 70 to 120%, with relative standard deviations from 7 to 34%. We detected roxarsone, its 3-amino and 3-acetamido metabolites, inorganic arsenic, and additional unknown arsenic species in livers from roxarsone-treated chickens. Both the originating laboratory and a second laboratory validated the method.
罗沙胂酸，（4-羟基-3-硝基苯）胂酸，是一种含砷化合物，自 20 世纪 40 年代以来已被批准作为家禽和猪的饲料添加剂；然而，对于可食组织中存在的残留砷形态，信息却很少。我们开发了一种从鸡肝中提取和定量砷形态的新方法。强堿溶液溶解肝脏，超滤除去大分子和颗粒物质。离子色谱将形态[亚砷酸盐、砷酸盐、一甲基胂酸、二甲基胂酸、（4-羟基-3-氨基苯）胂酸、（4-羟基-3-乙酰胺基苯）胂酸和罗沙胂酸]从提取物中分离出来，然后用电感耦合等离子体质谱法进行检测。提取过程中将大部分亚砷酸盐氧化为砷酸盐。对于添加浓度为 2μgkg(-1)及以上的情况，回收率在 70%至 120%之间，相对标准偏差在 7%至 34%之间。我们在罗沙胂酸处理过的鸡的肝脏中检测到了罗沙胂酸、它的 3-氨基和 3-乙酰胺代谢物、无机砷和其他未知的砷形态。原产实验室和第二个实验室都验证了该方法。
