Department of Plant Systematics, University of Bayreuth, D-95440 Bayreuth, Germany.
Am J Bot. 2004 Dec;91(12):1969-80. doi: 10.3732/ajb.91.12.1969.
Anther volatiles of 12 Ranunculaceae species distributed in six genera (Anemone, Aquilegia, Caltha, Pulsatilla, Ranunculus, and Trollius) were investigated by thermal desorption of anthers in quartz microvials inserted into a modified injector. Chemical analysis (GC-MS) yielded a total of 116 compounds from which 103 compounds could be identified. A great diversity of compounds was observed, including aliphatic compounds, aromatics, mono- and sesquiterpenoids, and nitrogen-bearing compounds. Intraspecific variation in the chemical profiles was significantly lower than interspecific variation. Eight compounds explained 96.1% of the observed total variation between the taxa: protoanemonin, octanal, (E,E)-α-farnesene, 2-phenyl ethanol, pentadecane, α-muurolene, phenyl acetaldehyde, and linalool oxide (pyranoid) were either present only in specific taxa or varied greatly in their relative amount between the taxa. An analysis of the scent data using the chord-normalized expected species shared (CNESS) distances of chemical profiles of the species, followed by visualization of the data with nonmetric multidimensional scaling (NMDS) showed that most species belonging to the same genus have similar chemical compositions. The differences in the chemical composition of anther volatiles are discussed with respect to the taxonomy and pollination biology of the species.
对 12 种毛茛科植物（银莲花属、耧斗菜属、金莲花属、白头翁属、毛茛属、翠雀属和侧金盏花属）的 12 种雄蕊的挥发物进行了研究，方法是将雄蕊插入石英微管中进行热解吸，然后插入改良的进样器中。化学分析（GC-MS）共得到 116 种化合物，其中 103 种可以鉴定。观察到化合物的多样性，包括脂肪族化合物、芳烃、单萜和倍半萜以及含氮化合物。种内化学图谱的变化明显低于种间变化。8 种化合物解释了 96.1%的观察到的分类群之间的总变异：原白头翁素、辛醛、（E，E）-α-法呢烯、2-苯乙醇、十五烷、α-穆勒烯、苯乙醛和芳樟醇氧化物（吡喃）要么仅存在于特定的分类群中，要么在分类群之间的相对含量差异很大。利用物种化学图谱的弦归一化预期共享物种（CNESS）距离对气味数据进行分析，然后使用非度量多维标度（NMDS）对数据进行可视化，结果表明，大多数属于同一属的物种具有相似的化学成分。讨论了雄蕊挥发物化学成分的差异与物种的分类学和传粉生物学的关系。
