Robert L, Godeau G, Gavignet-Jeannin C, Groult N, Six C, Robert A M
Laboratoire de Biochimie du Tissu Conjonctif, URA CNRS, 1174, Faculté de Médecine, Université Paris, Créteil, France.
Pathol Biol (Paris). 1990 Jun;38(6):608-16.
In normal conditions vascular permeability is precisely regulated by mechanisms which involve among others the macromolecules of extracellular matrix of the vascular wall. Permeability for a given substance will vary according to the anatomical localisation of the vessel determining also its structure and composition. In some pathological conditions, such as inflammation or diabetes, permeability can be abnormally increased. Increased permeability can be reproduced by i.v. collagenase injection. This permeability increase can be quantified by image analysis using appropriate tracers such as FITC-dextrans or horse-radish peroxidase, on histological sections from control and collagenase treated rats, pretreated or not with procyanidolic oligomers (PCO). We studied cerebral capillaries, aorta and cardiac muscle capillaries. It could be shown that previous treatment of animals with procyanidolic oligomers prevented the permeability increase produced by collagenase injection.
在正常情况下，血管通透性受到多种机制的精确调控，这些机制尤其涉及血管壁细胞外基质的大分子。给定物质的通透性会因血管的解剖位置而异，血管的解剖位置也决定了其结构和组成。在某些病理状况下，如炎症或糖尿病，通透性可能会异常增加。静脉注射胶原酶可导致通透性增加。使用适当的示踪剂，如异硫氰酸荧光素 - 葡聚糖或辣根过氧化物酶，通过图像分析对对照组和胶原酶处理组大鼠（无论是否用原花青素低聚物（PCO）预处理）的组织切片进行分析，可对这种通透性增加进行量化。我们研究了脑毛细血管、主动脉和心肌毛细血管。结果表明，用原花青素低聚物预先处理动物可防止胶原酶注射引起的通透性增加。
