Yucel Orhan, Ucar Ergun, Tozkoparan Ergun, Gunal Armagan, Akay Cemal, Sahin Mehmet Ali, Genc Onur
Department of Thoracic Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
J Cardiothorac Surg. 2009 Jul 28;4:40. doi: 10.1186/1749-8090-4-40.
We aimed to investigate the preventive effect of Proanthocyanidine (PC) in the prevention of RPE formation.
Subjects were divided into four groups each containing 10 rats. In the Control Group (CG): RPE wasn't performed. Then subjects were followed up for three days and they were sacrificed after the follow up period. Samplings were made from tissues for measurement of biochemical and histopathologic parameters. In the Second Group (PCG): The same protocol as CG was applied, except the administration of PC to the subjects. In the third RPE Group (RPEG): Again the same protocol as CG was applied, but as a difference, RPE was performed. In the Treatment Group (TG): The same protocol as RPEG was applied except the administration of PC to the subjects.
In RPEG group, the most important histopathological finding was severe pulmonary edema with alveolar damage and acute inflammatory cells. These findings were less in the TG group. RPE caused increased MDA levels, and decreased GPx, SOD and CAT activity significantly in lung tissue.
PC decreased MDA levels. Oxidative stress plays an important role in pathophysiology of RPE and PC treatment was shown to be useful to prevent formation of RPE.
我们旨在研究原花青素（PC）在预防放射性肺炎（RPE）形成方面的预防作用。
将实验对象分为四组，每组10只大鼠。对照组（CG）：未进行RPE。然后对实验对象进行三天的随访，并在随访期结束后处死。从组织中取样以测量生化和组织病理学参数。第二组（PCG）：应用与CG相同的方案，但给实验对象施用PC。在第三个RPE组（RPEG）：同样应用与CG相同的方案，但不同的是进行了RPE。治疗组（TG）：应用与RPEG相同的方案，但给实验对象施用PC。
在RPEG组中，最重要的组织病理学发现是伴有肺泡损伤和急性炎症细胞的严重肺水肿。这些发现在TG组中较少。RPE导致肺组织中丙二醛（MDA）水平升高，谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著降低。
PC降低了MDA水平。氧化应激在RPE的病理生理学中起重要作用，并且已证明PC治疗有助于预防RPE的形成。
