水芋（Dioscorea alata，薯蓣科）的新微卫星位点及其在其他薯蓣属物种中的交叉扩增。

New microsatellite loci for water yam (Dioscorea alata, Dioscoreaceae) and cross-amplification for other Dioscorea species.

机构信息

Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 83, Piracicaba, SP, Brazil, CEP 13400-970.

出版信息

Am J Bot. 2011 Jun;98(6):e144-6. doi: 10.3732/ajb.1000513. Epub 2011 May 27.

DOI:10.3732/ajb.1000513

PMID:21653503

Abstract

PREMISE OF THE STUDY

Dioscorea alata L. is one of the most widely distributed species of the genus in the humid and semihumid tropics and is associated with traditional agriculture. Only a few microsatellite markers have been developed so far for this and other Dioscorea species.

METHODS AND RESULTS

We isolated 14 codominant polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. Ten microsatellite loci were selected, and 80 D. alata accessions from different regions in Brazil were evaluated with nine polymorphic loci. The polymorphism information content (PIC) varied from 0.39 to 0.78 and the power discrimination (PD) ranged from 0.15 to 0.91. Six of the markers showed transferability for the species D. bulbifera, D. cayenensis-D. rotundata, and D. trifida.

CONCLUSIONS

The SSR markers obtained are an important tool for further studies aiming to characterize the genetic diversity in D. alata and other Dioscorea spp. accessions.

摘要

研究前提

盾叶薯蓣（Dioscorea alata L.）是薯蓣属中分布最广的物种之一，分布于湿润和半湿润热带地区，与传统农业相关。迄今为止，仅为该种和其他薯蓣属物种开发了少数微卫星标记。

方法和结果

我们使用微卫星富集基因组文库技术分离了 14 个共显性多态性微卫星标记。选择了 10 个微卫星位点，并用 9 个多态性位点评估了来自巴西不同地区的 80 个盾叶薯蓣样本。多态性信息含量（PIC）从 0.39 到 0.78 不等，有效鉴别率（PD）从 0.15 到 0.91 不等。其中 6 个标记在物种盾叶薯蓣、薯蓣（D. cayenensis-D. rotundata）和盾叶薯蓣（D. trifida）中具有可转移性。