Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 83, Piracicaba, SP, Brazil, CEP 13400-970.
Am J Bot. 2011 Jun;98(6):e144-6. doi: 10.3732/ajb.1000513. Epub 2011 May 27.
Dioscorea alata L. is one of the most widely distributed species of the genus in the humid and semihumid tropics and is associated with traditional agriculture. Only a few microsatellite markers have been developed so far for this and other Dioscorea species.
We isolated 14 codominant polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. Ten microsatellite loci were selected, and 80 D. alata accessions from different regions in Brazil were evaluated with nine polymorphic loci. The polymorphism information content (PIC) varied from 0.39 to 0.78 and the power discrimination (PD) ranged from 0.15 to 0.91. Six of the markers showed transferability for the species D. bulbifera, D. cayenensis-D. rotundata, and D. trifida.
The SSR markers obtained are an important tool for further studies aiming to characterize the genetic diversity in D. alata and other Dioscorea spp. accessions.
盾叶薯蓣（Dioscorea alata L.）是薯蓣属中分布最广的物种之一，分布于湿润和半湿润热带地区，与传统农业相关。迄今为止，仅为该种和其他薯蓣属物种开发了少数微卫星标记。
我们使用微卫星富集基因组文库技术分离了 14 个共显性多态性微卫星标记。选择了 10 个微卫星位点，并用 9 个多态性位点评估了来自巴西不同地区的 80 个盾叶薯蓣样本。多态性信息含量（PIC）从 0.39 到 0.78 不等，有效鉴别率（PD）从 0.15 到 0.91 不等。其中 6 个标记在物种盾叶薯蓣、薯蓣（D. cayenensis-D. rotundata）和盾叶薯蓣（D. trifida）中具有可转移性。
获得的 SSR 标记是进一步研究特征分析盾叶薯蓣和其他薯蓣属物种遗传多样性的重要工具。
