Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
Am J Bot. 2012 Oct;99(10):e425-7. doi: 10.3732/ajb.1200151. Epub 2012 Oct 1.
Microsatellite markers were developed in Dipteronia sinensis to investigate the population genetics of this endangered plant. •
Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 19 microsatellite loci were developed in D. sinensis and evaluated for their variability in 29 samples from a natural population. For the 15 polymorphic loci, the number of alleles ranged from nine to 33, while the observed and expected heterozygosities ranged from 0.3793 to 0.9655 and from 0.6029 to 0.9609, respectively. Their cross-taxa transferability was also investigated in Acer miaotaiense, A. palmatum, and A. pictum subsp. mono, and 10 to 15 loci proved amplifiable in these species. •
These microsatellite markers could be employed to investigate the population genetics of D. sinensis and may potentially be applicable to other related species.
开发双蕊兰的微卫星标记，以研究这种濒危植物的种群遗传学。
利用基于重复序列的 AFLP 快速分离（FIASCO）方案，在来自自然种群的 29 个样本中开发了 19 个微卫星位点，并评估了它们的变异性。对于 15 个多态性位点，等位基因数范围从 9 到 33，而观察到的和预期的杂合度分别为 0.3793 到 0.9655 和 0.6029 到 0.9609。还研究了它们在 Acer miaotaiense、A. palmatum 和 A. pictum subsp. mono 中的跨分类群可转移性，其中 10 到 15 个位点在这些物种中可扩增。
这些微卫星标记可用于研究双蕊兰的种群遗传学，并且可能潜在适用于其他相关物种。
