Chang Y N, Hsu H T, Hung M S, Chang C L, Li J G, Wang D L
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.
Chin J Physiol. 1990;33(1):31-40.
Prostaglandin E1 (PGE1) is frequently used as a stabilizing agent while isolating platelets. The platelet-stabilizing effect of PGE1 is mediate through the PGE1 receptor and the consequent increase of intracellular cAMP. The discoid shape of platelet is thus maintained. Recently, we have studied the effect of PGE1 on platelet deformability with micropipette aspiration technics, using pipettes with radii (Rp) of 0.26-0.36 micron. Platelets, obtained from platelet-rich plasma, and suspended in Hank's buffer containing BSA, glucose, adenosine and theophylline, were subjected for micropipette aspiration test within 2 hr after sampling. In general, PGE1 treated platelets (greater than 75%) remained as non-activated form as revealed under video microscopy and maintained their discoid shape for as long as 6 hr. The time course of the extension length (Dp, in micron) of the platelets in response to aspiration with a negative pressure of 5 cm H2O (tension = 0.15 dynes/cm) was analyzed by using a digital image analyzer system (IRIS 3120 with IP-512, with a resolution of 0.076 microns). A computer program based on the algorithm of real-time reading of image intensity was developed to facilitate real-time data acquisition. The data of Dp/Rp vs time (sec) were fitted by using a second-order equation. The deformability of PGE1 (0.1 microM) treated platelet was decreased significantly as compared with control. These results indicate that the platelet stabilization by PGE1 is accompanied by a decrease in platelet deformability.
前列腺素E1（PGE1）在分离血小板时常用作稳定剂。PGE1的血小板稳定作用是通过PGE1受体及细胞内cAMP的随之增加来介导的。从而维持了血小板的盘状形态。最近，我们用微吸管抽吸技术研究了PGE1对血小板变形性的影响，使用的吸管半径（Rp）为0.26 - 0.36微米。从富含血小板血浆中获取的血小板，悬浮于含有牛血清白蛋白、葡萄糖、腺苷和茶碱的汉克缓冲液中，在取样后2小时内进行微吸管抽吸试验。一般来说，经PGE1处理的血小板（超过75%）在视频显微镜下显示仍保持非活化形式，并能保持其盘状形态长达6小时。使用数字图像分析仪系统（带有IP - 512的IRIS 3120，分辨率为0.076微米）分析血小板在5 cm H2O负压（张力 = 0.15达因/厘米）抽吸下伸展长度（Dp，单位为微米）随时间的变化过程。开发了一个基于图像强度实时读取算法的计算机程序以促进实时数据采集。Dp/Rp与时间（秒）的数据用二阶方程拟合。与对照组相比，经0.1 microM PGE1处理的血小板变形性显著降低。这些结果表明，PGE1对血小板的稳定作用伴随着血小板变形性的降低。
