Komatsu S, Endo Y, Suzuki S
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Yakugaku Zasshi. 1990 May;110(5):325-31. doi: 10.1248/yakushi1947.110.5_325.
The changes of protein phosphorylation were analyzed during mouse sperm capacitation and acrosome reaction. Sperm adenosine 3',5'-cyclic monophosphate (cAMP) levels were gradually elevated during capacitation period. Although 32P uptake by sperm and protein phosphorylation increased during capacitation, the phosphorylation of a 45-kDa protein remained to be relatively constant. It was also demonstrated that acrosome reaction was induced by the treatment of capacitated sperm with 0.1 mM dibutyryl cAMP, 10 mM dibutyrylguanosine 3',5'-cyclic monophosphate, 20 microM calcium ionophore A23187 or acid-solubilized zonae pellucidae. The phosphorylation of a 45-kDa protein was augmented during the acrosome reaction induced by such agents. These results suggest that changes in protein phosphorylation might be involved in the regulation of mouse sperm capacitation and acrosome reaction.
在小鼠精子获能和顶体反应过程中分析了蛋白质磷酸化的变化。在获能期间，精子3',5'-环磷酸腺苷（cAMP）水平逐渐升高。尽管在获能过程中精子对32P的摄取和蛋白质磷酸化增加，但45 kDa蛋白质的磷酸化仍相对保持恒定。还证明，用0.1 mM二丁酰cAMP、10 mM二丁酰鸟苷3',5'-环磷酸、20 μM钙离子载体A23187或酸溶性透明带处理获能精子可诱导顶体反应。在这些试剂诱导的顶体反应过程中，45 kDa蛋白质的磷酸化增强。这些结果表明，蛋白质磷酸化的变化可能参与小鼠精子获能和顶体反应的调节。
