Savitz A J, Meyer D I
Department of Biological Chemistry, UCLA School of Medicine 90024.
Nature. 1990 Aug 9;346(6284):540-4. doi: 10.1038/346540a0.
Attachment of ribosomes to the membrane of the endoplasmic reticulum is one of the crucial first steps in the transport and secretion of intracellular proteins in mammalian cells. The process is mediated by an integral membrane protein of relative molecular mass 180,000 (Mr 180K), having a large (at least 160K) cytosolic domain that, when proteolytically detached from the membrane, can competitively inhibit the binding of ribosomes to intact membranes. Isolation of this domain has led to the identification, purification and characterization of the intact ribosome receptor, as well as its functional reconstitution into lipid vesicles.
核糖体附着于内质网膜是哺乳动物细胞内蛋白质运输和分泌过程中至关重要的起始步骤之一。该过程由一种相对分子质量为180,000（Mr 180K）的整合膜蛋白介导，该蛋白具有一个大的（至少160K）胞质结构域，当该结构域通过蛋白水解作用从膜上脱离时，能够竞争性抑制核糖体与完整膜的结合。分离该结构域已导致完整核糖体受体的鉴定、纯化和表征，以及其在脂质小泡中的功能重建。
