Phuc Pham Van, Ngoc Vu Bich, Lam Dang Hoang, Tam Nguyen Thanh, Viet Pham Quoc, Ngoc Phan Kim
Laboratory of Stem cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh, Vietnam.
Cell Tissue Bank. 2012 Jun;13(2):341-51. doi: 10.1007/s10561-011-9262-4. Epub 2011 Jun 8.
It is known that umbilical cord blood (UCB) is a rich source of stem cells with practical and ethical advantages. Three important types of stem cells which can be harvested from umbilical cord blood and used in disease treatment are hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). Since these stem cells have shown enormous potential in regenerative medicine, numerous umbilical cord blood banks have been established. In this study, we examined the ability of banked UCB collected to produce three types of stem cells from the same samples with characteristics of HSCs, MSCs and EPCs. We were able to obtain homogeneous plastic rapidly-adherent cells (with characteristics of MSCs), slowly-adherent (with characteristics of EPCs) and non-adherent cells (with characteristics of HSCs) from the mononuclear cell fractions of cryopreserved UCB. Using a protocol of 48 h supernatant transferring, we successfully isolated MSCs which expressed CD13, CD44 and CD90 while CD34, CD45 and CD133 negative, had typical fibroblast-like shape, and was able to differentiate into adipocytes; EPCs which were CD34, and CD90 positive, CD13, CD44, CD45 and CD133 negative, adherent with cobble-like shape; HSCs which formed colonies when cultured in MethoCult medium.
众所周知，脐带血是干细胞的丰富来源，具有实际和伦理优势。可以从脐带血中采集并用于疾病治疗的三种重要干细胞类型是造血干细胞（HSCs）、间充质干细胞（MSCs）和内皮祖细胞（EPCs）。由于这些干细胞在再生医学中已显示出巨大潜力，因此已经建立了许多脐带血库。在本研究中，我们检测了储存的脐带血从相同样本中产生具有造血干细胞、间充质干细胞和内皮祖细胞特征的三种干细胞的能力。我们能够从冷冻保存的脐带血的单核细胞部分获得均匀的塑料快速贴壁细胞（具有间充质干细胞特征）、缓慢贴壁细胞（具有内皮祖细胞特征）和非贴壁细胞（具有造血干细胞特征）。使用48小时上清液转移方案，我们成功分离出表达CD13、CD44和CD90而CD34、CD45和CD133阴性、具有典型成纤维细胞样形状且能够分化为脂肪细胞的间充质干细胞；CD34和CD90阳性、CD13、CD44、CD45和CD133阴性、呈卵石样贴壁的内皮祖细胞；以及在MethoCult培养基中培养时形成集落的造血干细胞。
