Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA.
Indoor Air. 2011 Dec;21(6):512-20. doi: 10.1111/j.1600-0668.2011.00728.x. Epub 2011 Jul 12.
We designed and tested a sampling and analysis system for quantitative measurement of airborne cockroach allergen with sufficient sensitivity for residential exposure assessment. Integrated 1-week airborne particle samples were collected at 10-15 LPM in 19 New York City apartments in which an asthmatic child who was allergic to cockroach allergen resided. Four simultaneous air samples were collected in each home: at heights of 0.3 and 1 m in the child's bedroom and in the kitchen. Extracts of air samples were analyzed by ELISA for the cockroach allergen Bla g2, modified by amplifying the colorimetric signal generated via use of AMPLI-Q detection system (DAKO Corporation, Carpinteria, CA, USA). Settled dust samples were quantified by conventional ELISA. Of the homes where cockroach allergen was detected in settled dust, Bla g2 also was detected in 87% and 93% of air samples in the bedroom and kitchen, respectively. Airborne Bla g2 levels were highly correlated within and between the bedroom and kitchen locations (P < 0.001). Expressed as picogram per cubic meter, the room average geometric mean for Bla g2 concentrations was 1.9 pg/m³ (95% CI 0.63, 4.57) and 3.8 pg/m³ (95% CI 1.35, 9.25) in bedrooms and kitchens, respectively. This method offers an attractive supplement to settled dust sampling for cockroach allergen exposure health studies.
Until now, cockroach allergen exposures have usually been assessed by collection and analysis of settled dust, on the assumption that airborne cockroach allergen cannot be reliably measured. In this study, a sensitive and quantitative method for measuring indoor airborne exposures to cockroach allergens involving a 7-day integrated total suspended particulate (TSP) sample collected at approximately 10-15 l/min was developed. Investigators are now empowered with an alternative exposure assessment method to supplement their studies and the understanding of allergen aerodynamics in the homes of children with asthma. We report airborne cockroach allergen in apartments, suggesting an ongoing burden of inhalation exposure.
设计并测试一种采样和分析系统，用于定量测量空气中的蟑螂过敏原，其灵敏度足以进行住宅暴露评估。
在纽约市 19 套公寓中，对 1 名患有哮喘且对蟑螂过敏原过敏的儿童进行了为期 1 周的集成式 1 周空气颗粒采样，采样流速为 10-15LPM。每个家庭同时采集 4 个空气样本：儿童卧室的 0.3 和 1m 处，以及厨房。使用 ELISA 法（通过 AMPLI-Q 检测系统（DAKO 公司，加利福尼亚州卡平特里亚）放大显色信号进行改良）分析空气样本中蟑螂过敏原 Bla g2 的含量。使用常规 ELISA 法对沉降尘样本进行定量分析。在有蟑螂过敏原沉降尘的家庭中，Bla g2 分别在卧室和厨房空气中样本中的检出率分别为 87%和 93%。卧室和厨房空气中 Bla g2 的水平高度相关（P<0.001）。以皮克/立方米表示，Bla g2 浓度的房间平均几何平均值分别为 1.9pg/m³（95%CI 0.63,4.57）和 3.8pg/m³（95%CI 1.35,9.25）。
这种方法为蟑螂过敏原暴露健康研究提供了一种有吸引力的沉降尘采样补充方法。
