Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
Virol J. 2011 Jun 9;8:290. doi: 10.1186/1743-422X-8-290.
Herpes simplex viruses exist as two major serotypes, type 1 (HSV-1) and type 2 (HSV-2). Determination of type, either HSV-1 or HSV-2, is important in accurate diagnosis and clinical control of transmission. Several tests are available for typing HSV, including a monoclonal antibody specific for glycoprotein G and several PCR assays.
A clinical isolate was identified as herpes simplex virus, but tested negative for both HSV-1 and HSV-2 antigens using type-specific monoclonal antibody assays. The isolate was determined to be HSV-1 by PCR analysis. A mutation which likely caused the monoclonal antibody non-reactivity was found in glycoprotein G. Phylogenetic analysis revealed two groups of HSV, one with the mutation and one without. Three population studies examining mutations in HSV-1 glycoprotein G were analyzed by chi-squared test. To this point, the epitope which the monoclonal antibody recognizes was only found in HSV-1 isolates from human European populations (p < 0.0001).
These findings suggest that the PCR-based methods for HSV typing may be more useful than the standard monoclonal antibody test in areas of the world where the variant in glycoprotein G is more prevalent.
单纯疱疹病毒存在两种主要血清型，即 1 型（HSV-1）和 2 型（HSV-2）。确定类型（HSV-1 或 HSV-2）对于准确诊断和临床控制传播非常重要。有几种用于疱疹病毒分型的检测方法，包括针对糖蛋白 G 的单克隆抗体和几种聚合酶链反应（PCR）检测。
临床分离株被鉴定为单纯疱疹病毒，但使用针对特定类型的单克隆抗体检测，HSV-1 和 HSV-2 抗原均呈阴性。该分离株通过 PCR 分析被确定为 HSV-1。在糖蛋白 G 中发现了一个可能导致单克隆抗体无反应性的突变。系统进化分析显示存在两组 HSV，一组有突变，一组没有。通过卡方检验分析了三项研究 HSV-1 糖蛋白 G 突变的人群研究。到目前为止，该单克隆抗体识别的表位仅在来自欧洲人群的 HSV-1 分离株中发现（p < 0.0001）。
这些发现表明，在糖蛋白 G 变异更为普遍的世界某些地区，基于 PCR 的 HSV 分型方法可能比标准的单克隆抗体检测更有用。
