Department of Chemistry, University of Calgary, Alberta Ingenuity Centre for Carbohydrate Science, Calgary, Canada.
Carbohydr Res. 2011 Sep 27;346(13):1692-704. doi: 10.1016/j.carres.2011.05.009. Epub 2011 May 14.
CST-II is a bacterial sialyltransferase known for its ability to perform α-(2→8)-sialylations using GM(3) related trisaccharide substrates. Previously, we probed the enzyme's substrate specificity and developed an efficient synthesis for α-(2→8)-oligosialosides, and we suggested that CST-II could have a very small substrate recognition domain. Here we report our full studies on CST-II's recognition feature for acceptor substrates. The current study further demonstrates the versatility of CST-II in preparing complex oligosaccharides that contain α-(2→8)-oligosialyl moieties.
CST-II 是一种细菌唾液酸转移酶，以使用 GM(3)相关三糖底物进行 α-(2→8)-唾液酸化的能力而闻名。此前，我们研究了该酶的底物特异性，并开发了一种有效的 α-(2→8)-寡唾液酸苷的合成方法，我们推测 CST-II 可能具有非常小的底物识别域。在这里，我们报告了我们对 CST-II 对受体底物识别特征的全面研究。本研究进一步证明了 CST-II 在制备含有 α-(2→8)-寡唾液酰基的复杂寡糖方面的多功能性。
