Rahman Q, Khan S G, Ali S
Industrial Toxicology Research Centre, Lucknow, India.
Chem Biol Interact. 1990;75(3):305-13. doi: 10.1016/0009-2797(90)90073-v.
The in vitro and in vivo effect of a carcinogenic variety of asbestos, chrysotile, both on xenobiotic metabolizing enzymes such as benzo[a]pyrene hydroxylase, epoxide hydrolase as well as glutathione-S-transferase activities and microsomal lipid peroxidation in rat lung were examined. The in vitro incubation of chrysotile with microsomes significantly adsorbed heme proteins, cytochrome P-450 and P-448 with the concomitant decrease in the dependent monooxygenase activities. The prolonged incubation of this mineral fibre with microsomes also resulted in the release of heme. It also led to the depletion in the activities of epoxide hydrolase and glutathione-S-transferase. However, it induced lipid peroxidation. When these in vitro effects were validated in vivo, the exposure to early stages produced similar alterations as observed in in vitro studies. However, reverse pattern in the alterations was observed after 90 days of exposure except in the case of lipid peroxidation which remained induced.
研究了致癌性石棉品种温石棉对大鼠肺中异源物代谢酶（如苯并[a]芘羟化酶、环氧化物水解酶以及谷胱甘肽-S-转移酶活性）和微粒体脂质过氧化的体内外效应。温石棉与微粒体的体外孵育显著吸附血红素蛋白、细胞色素P-450和P-448，同时依赖的单加氧酶活性降低。这种矿物纤维与微粒体的长时间孵育也导致血红素的释放。它还导致环氧化物水解酶和谷胱甘肽-S-转移酶活性的消耗。然而，它诱导脂质过氧化。当这些体外效应在体内得到验证时，早期暴露产生的变化与体外研究中观察到的相似。然而，暴露90天后观察到变化的模式相反，脂质过氧化除外，其仍被诱导。
