Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Org Biomol Chem. 2011 Jul 21;9(14):5051-5. doi: 10.1039/c1ob05540b. Epub 2011 Jun 9.
Intramolecular charge transfer (ICT) based fluorescent reagents containing a dansyl fluorophore have been synthesized and characterized. The reagent 1 and its complex, 1+Hg(2+) in sodium acetate buffer (pH 6.7) revealed considerable fluorescence enhancement (switched-on) in the presence of bovine serum albumin (BSA) with 10 ppb detection sensitivity. (1)H NMR spectral analysis suggests complexation between 1 and Hg(2+) ion involving the N,N-dimethylamino and carboxylic functions.
合成并表征了含有丹磺酰荧光团的分子内电荷转移（ICT）荧光试剂。在醋酸钠缓冲液（pH 6.7）中，试剂 1 及其与汞（II）离子的配合物 1+Hg(2+)在牛血清白蛋白（BSA）存在下显示出相当大的荧光增强（开启），检测灵敏度为 10 ppb。（1）H NMR 光谱分析表明，1 与 Hg(2+)离子的络合涉及 N,N-二甲基氨基和羧基官能团。
