State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, PR China.
Dalton Trans. 2011 Oct 14;40(38):9737-45. doi: 10.1039/c1dt10362h. Epub 2011 Aug 22.
A novel tetraazamacrocycle fluorescent sensor (6-(1-(dimethylamino)-5-naphthalene sulfonyl)-3,6,9,15-tetraazabicyclo[9.3.1] pentadeca-1(15),11,13-triene, 1) has been designed and prepared, which can be utilized for selective and ratiometric sensing of Hg(2+) and bovine serum albumin (BSA) with two different responsive modes in aqueous solution at physiological pH (50 mM Tris-HCl, pH 7.6). Above 0.5 ppb Hg(2+) can be discerned by coordination with 1 and the emission color changes enable 1 to be applied to a fast Hg(2+) test paper assay. Sensor 1 has also been demonstrated to be easily cell-penetrable and applicable for Hg(2+) imaging in living cells. Imaging of BSA in the gel using SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) stained in the medium containing 1 verified that the binding of 1 and BSA was successful in the presence of nonprotein substances. The linear range of 1 towards BSA utilizing ratiometric fluorescent calibration via noncovalent interaction in solution is 0-100 μg mL(-1) with a detection limit of 1 μg mL(-1), and has been successfully employed to determine the albumin concentration in blood serum by means of ratiometric fluorescent measurements for the first time. Finally, sensor 1 behaves as a fluorescent molecular switch composed of triple logic gates upon chemical inputs of Hg(2+) and BSA, which potentially provides intelligent diagnostics for Hg(2+) contaminated serum on the nanoscale.
一种新型的四氮杂大环荧光传感器（6-（1-（二甲基氨基）-5-萘磺酰基）-3,6,9,15-四氮杂双环[9.3.1]十五烷-1（15），11,13-三烯，1）已被设计和制备，该传感器可以在生理 pH 值（50mM Tris-HCl，pH7.6）的水溶液中以两种不同的响应模式用于选择性和比率检测 Hg(2+)和牛血清白蛋白（BSA）。与 1 配位后，可分辨出超过 0.5ppb 的 Hg(2+)，并且发射颜色的变化使 1 能够应用于快速 Hg(2+)试纸检测。传感器 1 还被证明很容易穿透细胞，适用于活细胞中的 Hg(2+)成像。在含有 1 的介质中用 SDS-PAGE（十二烷基硫酸钠聚丙烯酰胺凝胶电泳）对凝胶中的 BSA 进行染色成像，证明了在存在非蛋白质物质的情况下，1 与 BSA 的结合是成功的。在溶液中通过非共价相互作用进行比率荧光校准，1 对 BSA 的线性范围为 0-100μgmL(-1)，检测限为 1μgmL(-1)，并首次成功地用于通过比率荧光测量来确定血清白蛋白浓度。最后，传感器 1 表现为一个由三重逻辑门组成的荧光分子开关，通过化学输入 Hg(2+)和 BSA，这为纳米尺度上受 Hg(2+)污染的血清提供了智能诊断。
