Institut de Biologie et Chimie des Protéines, Université Lyon 1, Univ Lyon, CNRS FRE 3310, IFR128 BioSciences Gerland-Lyon Sud, 7 Passage du Vercors, 69367 Lyon Cedex 7, France.
Biotechnol Lett. 2011 Oct;33(10):2091-101. doi: 10.1007/s10529-011-0653-1. Epub 2011 Jun 10.
Adult mesenchymal stem cells (MSCs) are currently being investigated as an alternative to chondrocytes for repairing cartilage defects. As several collagen types participate in the formation of cartilage-specific extracellular matrix, we have investigated their gene expression levels during MSC chondrogenic induction. Bone marrow MSCs were cultured in pellet in the presence of BMP-2 and TGF-β3 for 24 days. After addition of FGF-2, at the fourth passage during MSC expansion, there was an enhancing effect on specific cartilage gene expression when compared to that without FGF-2 at day 12 in pellet culture. A switch in expression from the pre-chondrogenic type IIA form to the cartilage-specific type IIB form of the collagen type II gene was observed at day 24. A short-term addition of FGF-2 followed by a treatment with BMP-2/TGF-β3 appears sufficient to accelerate chondrogenesis with a particular effect on the main cartilage collagens.
成体间充质干细胞（MSCs）目前正在被研究作为修复软骨缺陷的软骨细胞替代物。由于几种胶原类型参与了软骨特异性细胞外基质的形成，我们研究了它们在 MSC 软骨诱导过程中的基因表达水平。骨髓间充质干细胞在 BMP-2 和 TGF-β3 的存在下在微球体中培养 24 天。在 MSC 扩增的第四代时，添加 FGF-2 后，与微球体培养的第 12 天不加 FGF-2 相比，对特定的软骨基因表达有增强作用。在第 24 天，观察到胶原 II 基因的从预软骨形成的 IIA 形式到软骨特异性的 IIB 形式的表达转换。短期添加 FGF-2 后再用 BMP-2/TGF-β3 处理似乎足以加速软骨形成，对主要的软骨胶原具有特殊的作用。
