Institut de Recherche pour le Développement, UMR 145, 911 avenue Agropolis, BP64501, 34394 Montpellier, France.
J Med Entomol. 2011 May;48(3):691-3. doi: 10.1603/me10183.
Although domestic animals may not be permissive for Plasmodium, they could nevertheless play a role in the epidemiology of malaria by attracting Anopheles away from humans. To investigate interactions between domestic animals and mosquitoes, we assayed immunoglobulin G (IgG) antibodies directed against the salivary proteins of Anopheles gambiae in domestic animals living in Senegalese villages where malaria is endemic. By Western blotting, sera from bovines (n=6), ovines (n=36), and caprines (n=36) did not react with Anopheles whole saliva. In contrast, equine sera recognized proteins in both saliva and salivary gland extracts. Two of the major immunogens (32 and 72 kDa) were also reactive in extracts from other major mosquito genera (Aedes and Culex), but reactions toAnopheles-specific antigens were detected in 12 of 17 horses. These data suggest that horses strongly react to Anopheles bites, and further experiments on horses are warranted to investigate the impact of this domestic animal species on the transmission of human malaria.
尽管家畜可能不会感染疟原虫，但它们可以通过吸引疟蚊远离人类，在疟疾的流行病学中发挥作用。为了研究家畜与蚊子之间的相互作用，我们检测了生活在塞内加尔疟疾流行地区的家畜针对冈比亚按蚊唾液蛋白的免疫球蛋白 G（IgG）抗体。通过 Western blot 分析，来自牛（n=6）、绵羊（n=36）和山羊（n=36）的血清与冈比亚按蚊全唾液均不发生反应。相比之下，马血清识别唾液和唾液腺提取物中的蛋白质。两种主要免疫原（32 和 72 kDa）也在其他主要蚊子属（伊蚊和库蚊）的提取物中反应，但在 17 匹马中有 12 匹检测到针对按蚊特异性抗原的反应。这些数据表明，马对按蚊叮咬有强烈反应，需要对马进行进一步实验，以研究这种家畜对人类疟疾传播的影响。
