Photolabile and paramagnetic derivatives of the nucleoside X and of Escherichia coli tRNAPhe. - Suppr | 超能文献

Photolabile and paramagnetic derivatives of the nucleoside X and of Escherichia coli tRNAPhe.

作者信息

Hansske F, Watanabe K, Cramer F, Seela F

出版信息

Hoppe Seylers Z Physiol Chem. 1978 Dec;359(12):1659-65. doi: 10.1515/bchm2.1978.359.2.1659.

DOI:10.1515/bchm2.1978.359.2.1659

Abstract

The synthesis of N3-[3-L-(5-azido-2-nitrobenzamido)-3-carboxypropyl]uridine (4b) and N3-[3-carboxy-3-L-(2,2,5,5-tetramethyl-3-pyrroline-3-carbonylamino)propyl]uridine Npyr-oxyl (4c) starting from the nucleoside X (4a) and the appropriate N-hydroxysuccinimide ester 1 or 2 is described. After acylation of tRNAPhe from E. coli (5a) with 1 or 2, the photolabile tRNAPhe derivative 5b and the paramagnetic tRNAPhe derivative 5c could be isolated. The position of modification in the polynucleotide chain was elucidated by comparison of the ribonuclease II/alkaline phosphatase digestion products of the substituted and unsubstituted tRNAPhe samples, and was identified as being exclusively the amino group of the nucleoside X in position 47 of E. coli tRNAPhe.

摘要

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Photolabile and paramagnetic derivatives of the nucleoside X and of Escherichia coli tRNAPhe.

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