转移核糖核酸分子的特异性自旋标记
Specific spin-labeling of transfer ribonucleic acid molecules.
作者信息
Caron M, Dugas H
出版信息
Nucleic Acids Res. 1976 Jan;3(1):19-34. doi: 10.1093/nar/3.1.19.
Abstract
The spin labels anhydride (ASL), bromoacetamide (BSL) and carbodiimide (CSL) were used to label selectively tRNAGlu, tRNA fMet and tRNAPhe from E. coli. The preparation and characterization of the sites of labeling of eight new spin-labeled tRNAs are described. The sites of labeling are: s2U using ASL, BSL and CLS and tRNAGlu; s4U using ASL and BSL on tRNAfMet and tRNAPhe; U-37 with CSL on tRNfMet; U-33 with CSL on tRNAPhe. The rare base X at position 47 of tRNAPhe has been acylated with a spin-labeled N-hydroxysuccinimide (HSL). The 3'end of unfractionated tRNA molecules has been chemically modified to a morpholino spin-labeled analogue (MSL). Their respective e.s.r. spectra are reported and discussed.
摘要
自旋标记酸酐（ASL）、溴乙酰胺（BSL）和碳二亚胺（CSL）被用于从大肠杆菌中选择性地标记tRNAGlu、tRNA fMet和tRNAPhe。本文描述了八种新的自旋标记tRNA标记位点的制备和表征。标记位点如下：使用ASL、BSL和CLS标记tRNAGlu的s2U；使用ASL和BSL标记tRNAfMet和tRNAPhe的s4U；使用CSL标记tRNfMet的U-37；使用CSL标记tRNAPhe的U-33。tRNAPhe第47位的稀有碱基X已被自旋标记的N-羟基琥珀酰亚胺（HSL）酰化。未分级的tRNA分子的3'端已被化学修饰为吗啉自旋标记类似物（MSL）。报告并讨论了它们各自的电子自旋共振光谱。
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