Gaudi S, Ponti W, Agresti A, Meneveri R, Malcovati M, Bonizzi L, Poli G, Amato A, Ginelli E
Dipartimento di Biologia e Genetica per le Scienze Mediche, Università di Milano, Italy.
Mol Cell Probes. 1990 Jun;4(3):163-74. doi: 10.1016/0890-8508(90)90050-a.
Classical serological methods and Southern blot hybridization for the diagnosis of bovine leukaemia virus (BLV) infection have been compared during the first nine months of life of offspring from BLV serum-negative and serum-positive dams belonging to a Friesian dairy herd in Italy. At birth, 9/13 calves analysed showed serum positivity for anti-gp60 BLV antibodies by agar immunodiffusion and/or by ELISA. However, only two calves were positive for BLV integrated proviruses in their lymphocyte DNA. At six months of age, anti-gp60 BLV antibodies and proviral DNA positivities were simultaneously shown only by the two cattle identified as DNA-positive at birth. This pattern remained constant up to nine months of age. Furthermore, analysis of the molecular characteristics of BLV integrated proviruses, carried out by using, as probes, the almost complete proviral genome (Belgian isolate) or a subclone of the env gene radioactively labelled or chemically modified, revealed that the calves under study were infected by a different isolate (Japanese isolate) and that, in one of the cattle, the majority of integrated proviruses was characterized by deletions probably located in the 5' half of the proviral genome.
在意大利一个弗里斯兰奶牛场中，对血清阴性和血清阳性的母畜所产后代生命的前九个月，比较了用于诊断牛白血病病毒（BLV）感染的经典血清学方法和Southern印迹杂交法。出生时，通过琼脂免疫扩散和/或ELISA分析的13头犊牛中有9头显示抗gp60 BLV抗体血清呈阳性。然而，只有两头犊牛的淋巴细胞DNA中BLV整合前病毒呈阳性。在六个月大时，仅出生时被鉴定为DNA阳性的两头牛同时显示抗gp60 BLV抗体和前病毒DNA呈阳性。这种模式一直持续到九个月大。此外，通过使用几乎完整的前病毒基因组（比利时分离株）或放射性标记或化学修饰的env基因亚克隆作为探针，对BLV整合前病毒的分子特征进行分析，结果显示所研究的犊牛感染的是不同的分离株（日本分离株），并且在其中一头牛中，大多数整合前病毒的特征是可能位于前病毒基因组5'端一半区域的缺失。
