Rossier J S, Roberts M A, Ferrigno R, Girault H H
Laboratoire d'Électrochimie, École Polytechnique Fédérale de Lausanne, 1015 CH-Lausanne, Switzerland.
Anal Chem. 1999 Oct 1;71(19):4294-9. doi: 10.1021/ac981382i.
A method, using UV laser photoablation, is presented for the fabrication and the integration of an electrochemical detector in a microchannel device, where carbon microband electrodes are placed either in the bottom or in the side walls of the rectangular microchannel. The different electrochemical cell geometries are tested with a model compound (ferrocenecarboxylic acid) in 40- and 100-μm-wide capillaries fabricated in planar polymer substrates. The experimental results are compared to numerical simulations for stagnant stream conditions. Depending on the scan rate and on the microchannel depth, the system behaves as a microband electrode until a linear diffusion field develops within the channel. The limit of detection for a one electron redox species within the 120-pL detection volume is ∼1 fmol with both cyclic voltammetry and chronoamperometric detection.
本文介绍了一种利用紫外激光光烧蚀技术在微通道器件中制造和集成电化学检测器的方法，其中碳微带电极放置在矩形微通道的底部或侧壁。在平面聚合物基板上制造的40微米和100微米宽的毛细管中，使用模型化合物（二茂铁羧酸）测试了不同的电化学池几何形状。将实验结果与停滞流条件下的数值模拟进行了比较。根据扫描速率和微通道深度，该系统在通道内形成线性扩散场之前表现为微带电极。在120皮升检测体积内，单电子氧化还原物质的循环伏安法和计时电流法检测的检测限约为1飞摩尔。
