噬菌体λ附着位点处DNA的弯曲与超螺旋化

Bending and supercoiling of DNA at the attachment site of bacteriophage lambda.

作者信息

Nash H A

机构信息

Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892.

出版信息

Trends Biochem Sci. 1990 Jun;15(6):222-7. doi: 10.1016/0968-0004(90)90034-9.

DOI:10.1016/0968-0004(90)90034-9

PMID:2166364

Abstract

Integration of the DNA of bacteriophage lambda into the chromosome of E. coli depends on the formation of a complex nucleoprotein array at a specific locus on the phage genome, the attachment site. Recent work shows how bending of this DNA (induced by a specific DNA-binding protein), and strain in this DNA (induced by supercoiling) contribute to the formation of the nucleoprotein structure. Further, there are new insights into the way this structure directs critical events during recombination.

摘要

噬菌体λ的DNA整合到大肠杆菌染色体中，依赖于在噬菌体基因组的特定位点（附着位点）形成复杂的核蛋白阵列。最近的研究表明，这种DNA的弯曲（由特定的DNA结合蛋白诱导）以及该DNA中的张力（由超螺旋诱导）如何有助于核蛋白结构的形成。此外，对于这种结构在重组过程中指导关键事件的方式有了新的见解。