Hsu P L, Ross W, Landy A
Nature. 1980 May 8;285(5760):85-91. doi: 10.1038/285085a0.
Site specific integrative recombination of bacteriophage lambda involves unequal partners. The minimal phage att site is composed of approximately 240-base pairs and four distinct binding sites for Int protein, at least three of which are crucial for function. This 'donor site' recombines efficiently with a smaller 'recipient site' that lacks the extensive interactions with Int protein.
噬菌体λ的位点特异性整合重组涉及不相等的伙伴。噬菌体最小的附着位点由大约240个碱基对和Int蛋白的四个不同结合位点组成,其中至少三个对功能至关重要。这个“供体位点”与一个较小的“受体位点”有效重组,该受体位点缺乏与Int蛋白的广泛相互作用。