Musculoskeletal Research Unit, Avon Orthopaedic Centre, Southmead Hospital, Bristol, United Kingdom.
Prostaglandins Other Lipid Mediat. 2011 Aug;95(1-4):45-52. doi: 10.1016/j.prostaglandins.2011.05.003. Epub 2011 May 31.
Lysophosphatidic acid (LPA), a pleiotropic signalling lipid is assuming growing significance in osteoblast biology. Although committed osteoblasts from several mammalian species are receptive to LPA far less is known about the potential for LPA to influence osteoblast formation from their mesenchymal progenitors. An essential factor for both bone development and post-natal bone growth and homeostasis is the active metabolite of vitamin D3, calcitriol (D3). Previously we reported how a combination of LPA and D3 synergistically co-operated to enhance the differentiation of immature human osteoblasts. Herein we provide evidence for the formation of human osteoblasts from multiple, primary human bone marrow derived stromal (stem) cells (hBMSCs). Importantly osteoblast development from hBMSCs only occurred when LPA was administered as a complex with albumin, its natural carrier. Collectively our findings support a co-operative role of LPA and D3 in osteoblastogenesis, findings which may aid the development of novel treatment strategies for bone repair.
溶血磷脂酸（LPA）是一种具有多种生物学功能的信号脂质分子，在成骨细胞生物学中具有重要意义。尽管来自几种哺乳动物的成骨细胞对 LPA 具有反应性，但对于 LPA 对其间充质祖细胞影响成骨细胞形成的潜力知之甚少。维生素 D3 的活性代谢物 1,25-二羟维生素 D3（calcitriol，D3）是骨发育和出生后骨生长及平衡的必需因子。我们之前曾报道过 LPA 和 D3 如何协同作用以增强未成熟人成骨细胞的分化。在此，我们提供了证据表明，人骨髓基质（干）细胞（hBMSCs）可以形成多种人成骨细胞。重要的是，只有当 LPA 与白蛋白（其天然载体）形成复合物时，hBMSCs 才会发育成成骨细胞。总的来说，我们的研究结果支持 LPA 和 D3 在成骨细胞发生中的协同作用，这一发现可能有助于开发骨修复的新治疗策略。
