King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia.
Nanomedicine. 2011 Dec;7(6):904-13. doi: 10.1016/j.nano.2011.04.011. Epub 2011 May 19.
Zinc oxide (ZnO) nanoparticles (NPs) are increasingly recognized for their utility in biological applications, including biosensor and medicine. However, little is known about the toxicity mechanisms of ZnO nanorods in human cells. This study was designed to investigate the possible mechanisms of apoptosis induced by ZnO nanorods in human alveolar adenocarcinoma (A549) cells. ZnO nanorod was found to induce cytotoxicity, reactive oxygen species (ROS) generation, oxidative stress and activities of caspase-3 & caspase-9 in a dose- and time-dependent manner. Western blot results showed that ZnO nanorods induced the expression of heat shock protein 70, a first-tier marker of cell damage and a cell-cycle checkpoint protein p53. Moreover, pro-apoptotic protein bax was upregulated and the antiapoptotic proteins, survivin and bcl-2, were downregulated in ZnO nanorod exposed cells. In conclusion, our data demonstrates that ZnO nanorod induced apoptosis in A549 cells through ROS and oxidative stress via p53, survivin, bax/bcl-2 and caspase pathways.
This study describes the mechanisms of apoptosis induced by ZnO nanorods in human alveolar adenocarcinoma cells.
氧化锌（ZnO）纳米粒子（NPs）因其在生物应用中的实用性而越来越受到关注，包括生物传感器和医学。然而，关于 ZnO 纳米棒在人类细胞中的毒性机制知之甚少。本研究旨在探讨 ZnO 纳米棒在人肺泡腺癌（A549）细胞中诱导细胞凋亡的可能机制。结果发现，ZnO 纳米棒以剂量和时间依赖的方式诱导细胞毒性、活性氧（ROS）生成、氧化应激和 caspase-3 和 caspase-9 的活性。Western blot 结果表明，ZnO 纳米棒诱导热休克蛋白 70（细胞损伤的一级标志物和细胞周期检查点蛋白 p53）的表达。此外，ZnO 纳米棒暴露的细胞中促凋亡蛋白 bax 上调，抗凋亡蛋白 survivin 和 bcl-2 下调。总之，我们的数据表明，ZnO 纳米棒通过 ROS 和氧化应激通过 p53、survivin、bax/bcl-2 和 caspase 途径诱导 A549 细胞凋亡。
本研究描述了 ZnO 纳米棒在人肺泡腺癌细胞中诱导细胞凋亡的机制。
