Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, MD 20742, USA.
Virology. 2011 Aug 15;417(1):113-25. doi: 10.1016/j.virol.2011.05.007. Epub 2011 Jun 12.
Circularization of cellular mRNAs is a key event prior to translation initiation. We report that efficient translation of Saguaro cactus virus (SCV) requires a 3' translational enhancer (PTE) located partially in coding sequences. Unlike a similar PTE reported in the 3' UTR of Pea enation mosaic virus that does not engage in an RNA:RNA interaction (Wang Z. et al., J. Biol. Chem. 284, 14189-14202, 2009), the SCV PTE participates in long distance RNA:RNA interactions with hairpins located in the p26 ORF and in the 5' UTR of one subgenomic RNA. At least two additional RNA:RNA interactions are also present, one of which involves the p26 initiation codon. Similar PTE can be found in six additional carmoviruses that can putatively form long-distance interactions with 5' hairpins located in comparable positions.
环状化细胞 mRNA 是翻译起始前的关键事件。我们报告称，仙人掌病毒（SCV）的有效翻译需要一个位于编码序列部分的 3'翻译增强子（PTE）。与在豌豆花叶病毒 3'UTR 中报道的不参与 RNA:RNA 相互作用的类似 PTE 不同（Wang Z. 等人，J. Biol. Chem. 284, 14189-14202, 2009），SCV PTE 与位于 p26 ORF 和一个亚基因组 RNA 的 5'UTR 中的发夹参与长距离 RNA:RNA 相互作用。至少还有另外两个 RNA:RNA 相互作用，其中一个涉及 p26 起始密码子。在另外六个推定可与位于类似位置的 5'发夹形成长距离相互作用的 carmoviruses 中也可以找到类似的 PTE。
