Draper K G, Ceruzzi M, Kmetz M E, Sturzenbecker L J
Department of Antiviral Chemotherapy, Schering-Plough Corporation, Bloomfield, NJ 07003.
Antiviral Res. 1990 Apr;13(4):151-64. doi: 10.1016/0166-3542(90)90034-5.
The virion tegument protein, Vmw65, of herpes simplex virus is a transacting molecule which induces immediate early gene transcription. We show that an oligodeoxyribonucleotide which is complementary to the translation initiation region of Vmw65 mRNA inhibited the expression of Vmw65 biological activity in a Vmw65-expressing cell line and reduced the yield of HSV-1 in tissue culture. The levels of oligomer required to effect viral replication resulted in no observable cellular toxicity.
单纯疱疹病毒的病毒体被膜蛋白Vmw65是一种反式作用分子，可诱导立即早期基因转录。我们发现，一种与Vmw65 mRNA翻译起始区域互补的寡脱氧核糖核苷酸，在表达Vmw65的细胞系中抑制了Vmw65生物活性的表达，并降低了组织培养中HSV-1的产量。影响病毒复制所需的寡聚物水平未导致可观察到的细胞毒性。
