hDlg 和 PTEN 在癌前病变和宫颈癌中的异常分布。
Abnormal distribution of hDlg and PTEN in premalignant lesions and invasive cervical cancer.
机构信息
Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología/Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico.
出版信息
Gynecol Oncol. 2011 Sep;122(3):663-8. doi: 10.1016/j.ygyno.2011.05.017. Epub 2011 Jun 12.
OBJECTIVE
The aim of the present study was to evaluate differences in expression levels and localization status of PTEN, p53 and hDlg suppressor proteins in premalignant lesions and cervical cancer, and to analyze the possible correlation between them.
METHODS
Expression levels (positivity/intensity) and localization (nuclear, membrane or cytoplasmic) of PTEN, hDlg and p53 were analyzed by immunohistochemistry in 43 cases with different stages of cervical intraepithelial neoplasia (CIN) and 105 invasive cervical carcinomas (ICC) (91 squamous carcinoma, 14 adenocarcinoma). Differences between proportions were evaluated.
RESULTS
We found a decreased expression of PTEN in ICC that correlated with a loss of hDlg from the cell membrane. In contrast, no changes were found in p53 protein levels or localization in CIN and ICC.
CONCLUSIONS
These results suggest that the abnormal expression and localization of PTEN during cervical carcinogenesis may be a consequence of modifications in the expression patterns of hDlg.
目的
本研究旨在评估抑癌基因 PTEN、p53 和 hDlg 抑制蛋白在癌前病变和宫颈癌中的表达水平和定位状态的差异，并分析它们之间的可能相关性。
方法
采用免疫组织化学法检测 43 例不同宫颈上皮内瘤变（CIN）和 105 例浸润性宫颈癌（ICC）（91 例鳞癌，14 例腺癌）组织中 PTEN、hDlg 和 p53 的表达水平（阳性率/强度）和定位（核、膜或细胞质）。评估比例差异。
结果
我们发现 ICC 中 PTEN 的表达降低，与 hDlg 从细胞膜丢失相关。相比之下，CIN 和 ICC 中 p53 蛋白水平或定位没有变化。
结论
这些结果表明，PTEN 在宫颈癌发生过程中的异常表达和定位可能是 hDlg 表达模式改变的结果。
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