Departamento de Zoología y Antropología Física, Facultad de Biología, Universidad Complutense, Ciudad Universitaria, 28040 Madrid, Spain.
Forensic Sci Int Genet. 2012 Mar;6(2):e66-71. doi: 10.1016/j.fsigen.2011.05.007. Epub 2011 Jun 12.
Seventeen Y-chromosomal short tandem repeats (STRs) were analyzed in 347 healthy, unrelated, autochthonous males from the Andalusian provinces of Huelva (N=167) and Granada (N=180). AmpFlSTR Y-filer PCR Amplification kit (Applied Biosystems) was used to type the Y-STR markers. A total of 156 and 166 different haplotypes for the 17 Y-STR set were detected in Huelva, and Granada, respectively. The same haplotype diversity was found for both samples (0.998±0.001), and the overall discrimination capacity was 0.904. The most common minimal haplotype (DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393) in both subpopulations was 14-13-16-24-11-13-13, which is also the most frequent haplotype among Atlantic European populations. Comparison analysis using pairwise R(ST) values and Analysis of Molecular Variance (AMOVA) revealed a significant genetic distance between our Andalusian samples and other ones from the northern Iberian fringe (including Basque and Pyrenean populations). However, results from the multi-dimensional scaling analysis (MDS) yielded a well-defined group of Iberian populations separated from the other Mediterranean clusters observed.
对来自安达卢西亚的 347 名健康、无关、土生土长的男性（赫雷斯省 167 名，格拉纳达省 180 名）的 17 个 Y 染色体短串联重复序列（STR）进行了分析。使用 AmpFlSTR Y-filer PCR 扩增试剂盒（Applied Biosystems）对 Y-STR 标记进行分型。在赫雷斯和格拉纳达，分别检测到 17 个 Y-STR 组中的 156 种和 166 种不同的单倍型。两个样本的相同单倍型多样性（0.998±0.001），总体鉴别能力为 0.904。两个亚群中最常见的最小单倍型（DYS19、DYS389 I、DYS389 II、DYS390、DYS391、DYS392、DYS393）为 14-13-16-24-11-13-13，这也是大西洋欧洲人群中最常见的单倍型。使用成对 R(ST)值和分子方差分析（AMOVA）进行的比较分析显示，我们的安达卢西亚样本与伊比利亚北缘的其他样本（包括巴斯克和比利牛斯人群）之间存在显著的遗传距离。然而，多维尺度分析（MDS）的结果显示，伊比利亚人群形成了一个明确的群体，与其他地中海群体分离。
