Institute of Health Science, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences-Shanghai Jiao-Tong University School of Medicine, Shanghai, China.
FEBS Lett. 2011 Jul 21;585(14):2307-12. doi: 10.1016/j.febslet.2011.05.061. Epub 2011 Jun 12.
Besides the contribution in cell death, apoptosis-inducing factor (AIF) also exerts roles in other cellular activities, which are largely unknown. The CCAAT-enhancer binding protein alpha (C/EBPα) is required for differentiation of adipocytes and granulocytes. Here we report that, during 3T3-L1 adipocyte differentiation, AIF expression is robustly upregulated via transcriptional regulation by C/EBPα. The upregulated effect is also confirmed by knockdown and ectopic expression of C/EBPα in U937 and MCF-7 cells respectively with and without endogenous expression of C/EBPα protein. We also reveal that AIF knockdown attenuates 3T3-L1 adipocyte differentiation, presumably due to the mitochondrial respiratory chain deficiency. These results provide evidence for the role of AIF in adipocyte differentiation.
除了在细胞死亡中的作用外，凋亡诱导因子（AIF）在其他细胞活动中也发挥作用，但这些作用在很大程度上尚不清楚。CCAAT 增强子结合蛋白α（C/EBPα）是脂肪细胞和粒细胞分化所必需的。在这里，我们报告在 3T3-L1 脂肪细胞分化过程中，AIF 的表达通过 C/EBPα 的转录调控得到强烈上调。通过分别在具有和不具有内源性 C/EBPα 蛋白表达的 U937 和 MCF-7 细胞中敲低和过表达 C/EBPα，也证实了上调作用。我们还揭示 AIF 敲低可减弱 3T3-L1 脂肪细胞分化，这可能是由于线粒体呼吸链缺陷所致。这些结果为 AIF 在脂肪细胞分化中的作用提供了证据。
