在体内β-肾上腺素能受体刺激过程中，两种新型c-abl mRNA在大鼠腮腺唾液腺中表达。

Two novel c-abl mRNAs are expressed in rat parotid salivary glands during in vivo beta-adrenergic receptor stimulation.

作者信息

Mertz P M, Backman T, Bernards A, Kousvelari E

机构信息

Clinical Investigations and Patient Care Branch, National Institute of Dental Research, NIH, Bethesda, MD 20832.

出版信息

Biochim Biophys Acta. 1990 Jul 30;1049(3):272-7. doi: 10.1016/0167-4781(90)90097-l.

DOI:10.1016/0167-4781(90)90097-l

PMID:2166578

Abstract

The c-abl proto-oncogene is transcribed in most cell lines and tissues into two mRNAs of 6.5 and 5.3 kb, which have different 5' ends and encode two 150 kDa proteins that are largely colinear, but have different N-termini. We show here that two unusually short and abundant c-abl-related mRNAs of 1.5 and 1.3 kb appear in rat parotid salivary glands, within 1 day of in vivo administration of the beta-adrenergic receptor agonist isoproterenol. These transcripts are not found in the submandibular salivary gland or in the heart and they are too short to encode the known c-abl proteins. RNA blot, S1 nuclease protection and primer extension analysis suggest that the isoproterenol inducible parotid gland mRNAs do not contain the kinase domain, but represent part of the C-terminal segment of the abl reading frame.

摘要

原癌基因c-abl在大多数细胞系和组织中转录成6.5 kb和5.3 kb的两种mRNA，它们具有不同的5'端，编码两种150 kDa的蛋白质，这两种蛋白质在很大程度上是共线性的，但N端不同。我们在此表明，在体内给予β-肾上腺素能受体激动剂异丙肾上腺素1天内，大鼠腮腺唾液腺中出现了两种异常短且丰富的1.5 kb和1.3 kb的与c-abl相关的mRNA。在下颌下唾液腺或心脏中未发现这些转录本，并且它们太短而无法编码已知的c-abl蛋白。RNA印迹、S1核酸酶保护和引物延伸分析表明，异丙肾上腺素诱导的腮腺mRNA不包含激酶结构域，而是代表abl阅读框C端片段的一部分。