Kousvelari E, Louis J M, Huang L H, Curran T
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, Maryland 20892.
Exp Cell Res. 1988 Nov;179(1):194-203. doi: 10.1016/0014-4827(88)90358-8.
Stimulation of beta-adrenoreceptors in rat parotid acinar cells in vitro by the beta-adrenergic agonist isoproterenol induces steady-state levels of c-fos mRNA and c-fos protein in these cells. A dramatic increase in the steady-state levels of c-fos mRNA was observed at 60 min, followed by a decrease at 2 h with a second peak at 4 h. c-fos induction in rat parotid acinar cells in vitro seems to be mediated by cAMP. Increased levels of p53 and c-myc mRNA were detected only at 60 min. c-abl and c-sis were also induced by isoproterenol but in a pattern different from that seen with c-fos. c-abl was the only oncogene in rat parotid gland which showed increased expression after chronic isoproterenol treatment of rats. In rat parotid acinar cells we observed no correlation between DNA synthesis and c-fos induction.
在体外，β - 肾上腺素能激动剂异丙肾上腺素刺激大鼠腮腺腺泡细胞中的β - 肾上腺素受体，可诱导这些细胞中c - fos mRNA和c - fos蛋白达到稳态水平。在60分钟时观察到c - fos mRNA的稳态水平急剧增加，随后在2小时时下降，并在4小时出现第二个峰值。体外大鼠腮腺腺泡细胞中c - fos的诱导似乎由cAMP介导。仅在60分钟时检测到p53和c - myc mRNA水平升高。c - abl和c - sis也被异丙肾上腺素诱导，但模式与c - fos不同。c - abl是大鼠腮腺中唯一在大鼠经慢性异丙肾上腺素处理后表达增加的癌基因。在大鼠腮腺腺泡细胞中，我们未观察到DNA合成与c - fos诱导之间的相关性。
