Department of Pharmaceutical Sciences, University of Padua, Via Marzolo 5, I-35131 Padua, Italy.
Int J Mol Med. 2011 Sep;28(3):315-25. doi: 10.3892/ijmm.2011.720. Epub 2011 Jun 8.
The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.
本研究旨在探讨脱细胞方案对小直径(<6mm)胫腓骨血管结构和力学性能的影响。采用去污剂-酶法(DEM)对小牛血管进行脱细胞处理，部分用胰蛋白酶水解，然后用聚乙二醇二缩水甘油醚交联。结果表明：i)DEM 可被视为一种简单有效的制备完整去细胞动脉和静脉的方法，能很好地保留高比例的胶原和弹性纤维；ii)聚乙二醇二缩水甘油醚交联处理为血管提供了适当的力学增强。组织学上，采用去污剂-酶法处理可获得脱细胞血管，其天然细胞外基质组织形态和成分得以很好地保留。此外，该脱细胞方案可有效去除小直径胫腓骨血管的 HLA Ⅰ类抗原表达。通过分别在血管腔面和外膜面接种内皮细胞和平滑肌细胞，评估了脱细胞交联血管的细胞相容性。
