Scatton B, Benavides J, Dubois A, Bourdiol F
Biology Department, Synthélabo Recherche (L.E.R.S.), Bagneux, France.
Int J Tissue React. 1990;12(1):15-20.
The effects of chronic inflammatory lesions, induced by local injection of Freund's adjuvant into the rat hind limb, on omega 3 (peripheral type benzodiazepine) binding sites in the immune organs and leg infiltrate have been studied by autoradiography, using the specific photoaffinity ligand for omega 3 sites, 3H-PK 14105. In these arthritic rats, omega 3-site density in the thymus was about 2-fold greater than the values from control animals. Binding increases were similar in magnitude in the medulla and cortex. In contrast, omega 3 binding levels remained unchanged in the spleen. In the hyperplastic iliac lymph nodes, the morphological changes were accompanied by an increase in omega 3-site density in the proliferative centres and secondary follicles. In the inflammatory infiltrate of the leg an accumulation of omega 3-associated autoradiographic grains was observed over large multinucleated cells (possibly LE cells of Hargraves) and small thymocyte-like cells. Granulocyte-like cells located in the lumen of the vessels in the inflammatory lymph nodes and leg muscular infiltrate also had high amounts of autoradiographic grains. These findings demonstrate that in the inflammatory processes subsequent to the local injection of Freund's adjuvant, the activation of the immune system results in an increase in omega 3-site densities in immune organs and inflammatory lesions. This observation may be relevant to the proposed immunomodulatory role of these binding sites.
通过放射自显影技术，使用针对ω3位点的特异性光亲和配体3H-PK 14105，研究了在大鼠后肢局部注射弗氏佐剂诱导的慢性炎性病变对免疫器官和腿部浸润物中ω3（外周型苯二氮䓬）结合位点的影响。在这些关节炎大鼠中，胸腺中ω3位点密度比对照动物的值大约高2倍。髓质和皮质中的结合增加幅度相似。相比之下，脾脏中的ω3结合水平保持不变。在增生的髂淋巴结中，形态学变化伴随着增殖中心和次级滤泡中ω3位点密度的增加。在腿部的炎性浸润物中，在大型多核细胞（可能是哈格雷夫斯的LE细胞）和小型胸腺细胞样细胞上观察到与ω3相关的放射自显影颗粒的积累。位于炎性淋巴结血管腔和腿部肌肉浸润物中的粒细胞样细胞也有大量放射自显影颗粒。这些发现表明，在局部注射弗氏佐剂后的炎症过程中，免疫系统的激活导致免疫器官和炎性病变中ω3位点密度增加。这一观察结果可能与这些结合位点所提出的免疫调节作用有关。
