Spargo L D, Hawkes J S, Cleland L G, Mayrhofer G
Arthritis Research Laboratory, The Hanson Centre for Cancer Research, Adelaide, Australia.
J Immunol. 1996 Dec 1;157(11):5198-207.
Recruitment of [125I]iododeoxyuridine-labeled syngeneic lymphoblasts from thoracic duct (TD) lymph into periarticular tissues has been examined after intravenous administration to normal rats and to rats with adjuvant-induced arthritis. Uptake of label was observed in the inflamed paws of arthritic rats and cells were located in synovium and periarticular bone marrow by autoradiography. Uptake was greater with lymphoblasts from donors in the late prodromal phase of adjuvant-induced arthritis (arthritic donors) than from normal donors. With arthritic donors, recruitment of lymphoblasts from TD lymph was greater than from mesenteric duct lymph, suggesting that most of the joint-seeking lymphoblasts in arthritic rats arose in peripheral lymphoid tissues. Lymphoblasts from arthritic donors were also detected in the synovium of paws from normal rats. Recovery of lymphoblasts was monitored in other tissues; this revealed, in arthritic recipients, competition among extra-articular sites of inflammation (adjuvant injection site, draining lymph nodes, and lymph nodes draining affected joints), the lungs, and the inflamed synovium for recruitment of lymphoblasts from arthritic donors. In contrast, while some lymphoblasts from normal donors were recruited to inflamed joints, the small intestine was the main site of recruitment. The results reflect the known propensity of T lymphoblasts generated in peripheral lymphoid tissues to enter inflamed tissues. However, some mesenteric duct lymphoblasts also entered inflamed synovium. The observed pattern of recruitment of lymphoblasts to synovium is pertinent to the pathogenesis of arthritis, the potential roles of arthritogenic and "bystander" lymphocytes and the known links between the joints and inflammation in the intestine.
在对正常大鼠和佐剂诱导性关节炎大鼠静脉注射后，研究了[125I]碘脱氧尿苷标记的同基因淋巴母细胞从胸导管（TD）淋巴进入关节周围组织的情况。在关节炎大鼠的炎症爪子中观察到标记物的摄取，通过放射自显影法确定细胞位于滑膜和关节周围骨髓中。与来自正常供体的淋巴母细胞相比，来自佐剂诱导性关节炎前驱期晚期供体（关节炎供体）的淋巴母细胞摄取量更大。对于关节炎供体，来自TD淋巴的淋巴母细胞募集量大于来自肠系膜导管淋巴的募集量，这表明关节炎大鼠中大多数寻找关节的淋巴母细胞产生于外周淋巴组织。在正常大鼠爪子的滑膜中也检测到了来自关节炎供体的淋巴母细胞。在其他组织中监测了淋巴母细胞的恢复情况；这表明，在关节炎受体中，关节外炎症部位（佐剂注射部位、引流淋巴结和受影响关节的引流淋巴结）、肺和炎症滑膜之间存在竞争，以募集来自关节炎供体的淋巴母细胞。相比之下，虽然一些来自正常供体的淋巴母细胞被募集到炎症关节，但小肠是主要的募集部位。这些结果反映了外周淋巴组织中产生的T淋巴母细胞进入炎症组织的已知倾向。然而，一些肠系膜导管淋巴母细胞也进入了炎症滑膜。观察到的淋巴母细胞向滑膜募集的模式与关节炎的发病机制、致关节炎淋巴细胞和“旁观者”淋巴细胞的潜在作用以及关节与肠道炎症之间的已知联系相关。
