Bio-Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan.
J Fluoresc. 2011 Nov;21(6):2057-68. doi: 10.1007/s10895-011-0904-5. Epub 2011 Jun 14.
The use of fluorescent nanomaterials with good photostability and biocompatibility in live imaging of cells has gained increased attention. Even though several imaging techniques have been reported for mammalian cells, very limited literatures are available for nanomaterial based live imaging in plant system. We studied the uptake ability of two different nanomaterials, the highly photostable CdSe quantum dots and highly biocompatible FITC-labeled silica nanoparticles by rice seedlings which could provide greater opportunities for developing novel in vivo imaging techniques in plants. The effects of these nanomaterials on rice seed germination have also been studied for analyzing their phytotoxic effects on plants. We observed good germination of seeds in the presence of FITC-labeled silica nanoparticles whereas germination was arrested with quantum dots. The uptake of both the nanomaterials has been observed with rice seedlings, which calls for more research for recommending their safe use as biolabels in plants.
在细胞的活体成像中，使用具有良好光稳定性和生物相容性的荧光纳米材料已经引起了越来越多的关注。尽管已经报道了几种用于哺乳动物细胞的成像技术，但基于纳米材料的植物系统活体成像的文献非常有限。我们研究了两种不同纳米材料的摄取能力，即高度稳定的 CdSe 量子点和高度生物相容的 FITC 标记的硅纳米粒子，这为开发植物体内新型活体成像技术提供了更多机会。我们还研究了这些纳米材料对水稻种子萌发的影响，以分析它们对植物的植物毒性作用。我们观察到在 FITC 标记的硅纳米粒子存在下，种子具有良好的萌发能力，而量子点则阻止了种子的萌发。我们观察到水稻幼苗对这两种纳米材料的摄取，这需要进一步研究，以推荐它们作为植物生物标记物的安全使用。
