Xu J A, Hsu C Y, Liu T H, Hogan E L, Perot P L, Tai H H
Department of Neurology, Medical University of South Carolina, Charleston 29425.
J Neurochem. 1990 Sep;55(3):907-12. doi: 10.1111/j.1471-4159.1990.tb04577.x.
Activation of arachidonic acid occurs after spinal cord injury. Leukotriene B4 is a lipoxygenase metabolite of arachidonic acid. In a rat model of experimental spinal cord injury, we found that the leukotriene B4 content was less than the sensitivity of our assay (8 pg/mg of protein) in non-traumatized spinal cord. Leukotriene B4 was detectable in traumatized cord (mean +/- SE, 25 +/- 5 pg/mg of protein; n = 3). Release of leukotriene B4 from spinal cord slices into the incubation medium was also noted after trauma (9 +/- 1 pg/mg of protein; n = 12) and was enhanced by exposure of traumatized spinal cord slices to the calcium ionophore A23187 (375 +/- 43 pg/mg of protein; n = 12). The amount of leukotriene B4 released corresponded to the extent of post-traumatic polymorphonuclear cell infiltration determined by a myeloperoxidase assay. Results from this study suggest that the source of leukotriene B4 in spinal cord injury is infiltrating polymorphonuclear cells.
花生四烯酸的激活发生在脊髓损伤后。白三烯B4是花生四烯酸的一种脂氧合酶代谢产物。在实验性脊髓损伤的大鼠模型中，我们发现，在未受损伤的脊髓中，白三烯B4的含量低于我们检测方法的灵敏度（8 pg/mg蛋白质）。在受损伤的脊髓中可检测到白三烯B4（平均值±标准误，25±5 pg/mg蛋白质；n = 3）。创伤后还观察到白三烯B4从脊髓切片释放到孵育培养基中（9±1 pg/mg蛋白质；n = 12），并且通过将受损伤的脊髓切片暴露于钙离子载体A23187而增强（375±43 pg/mg蛋白质；n = 12）。释放的白三烯B4的量与通过髓过氧化物酶测定法确定的创伤后多形核细胞浸润程度相对应。这项研究的结果表明，脊髓损伤中白三烯B4的来源是浸润的多形核细胞。
