Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Viral Immunol. 2011 Jun;24(3):179-87. doi: 10.1089/vim.2010.0125.
Respiratory epithelial cells are one of main targets for infections caused by influenza viruses. Recently, the induction of proinflammatory cytokines and toll-like receptors (TLRs) in normal human bronchial/tracheal epithelial cells infected with seasonal H1N1, 2009 pandemic H1N1, seasonal H3N2, or highly pathogenic H5N1 influenza virus were studied to understand the pathogenesis and early immune responses. The cells were productively infected with the viruses. Among the inflammatory cytokines tested, interleukin (IL)-8 was predominantly induced in virus-infected cells. Among the chemokines tested, interferon-γ-inducible protein-10 (IP-10) and growth-related oncogene-α (GRO-α) were predominantly induced in virus-infected cells. TLR-5 was predominantly induced in cells infected with seasonal H1N1, pandemic H1N1, or H5N1 influenza virus, and TLR-3 was predominantly induced in cells infected with seasonal H3N2 influenza virus. Taken together, the results suggest that IL-8, IP-10, and GRO-α are predominantly induced in respiratory epithelial cells infected with influenza A viruses, and that TLR-5 and TLR-3 are involved in the stimulation of virus-infected respiratory epithelial cells.
呼吸道上皮细胞是流感病毒感染的主要靶细胞之一。最近，研究了季节性 H1N1、2009 年大流行 H1N1、季节性 H3N2 或高致病性 H5N1 流感病毒感染正常人支气管/气管上皮细胞后诱导产生的促炎细胞因子和 Toll 样受体（TLR），以了解发病机制和早期免疫反应。细胞被病毒有效感染。在所测试的炎症细胞因子中，白细胞介素（IL）-8 在病毒感染的细胞中被强烈诱导。在所测试的趋化因子中，干扰素-γ诱导蛋白-10（IP-10）和生长相关癌基因-α（GRO-α）在病毒感染的细胞中被强烈诱导。TLR-5 在感染季节性 H1N1、大流行 H1N1 或 H5N1 流感病毒的细胞中被强烈诱导，TLR-3 在感染季节性 H3N2 流感病毒的细胞中被强烈诱导。总之，结果表明，IL-8、IP-10 和 GRO-α 在感染甲型流感病毒的呼吸道上皮细胞中被强烈诱导，TLR-5 和 TLR-3 参与了病毒感染的呼吸道上皮细胞的刺激。
