牛磺酸对离体大鼠肝细胞钙及氧反常的钙相关细胞保护作用。

Calcium-associated cytoprotective effect of taurine on the calcium and oxygen paradoxes in isolated rat hepatocytes.

作者信息

Nakashima T, Seto Y, Nakajima T, Shima T, Sakamoto Y, Cho N, Sano A, Iwai M, Kagawa K, Okanoue T

机构信息

Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan.

出版信息

Liver. 1990 Jun;10(3):167-72. doi: 10.1111/j.1600-0676.1990.tb00453.x.

DOI:10.1111/j.1600-0676.1990.tb00453.x

PMID:2166891

Abstract

Our study was designed to determine the calcium and oxygen paradoxes in isolated rat hepatocytes, and to evaluate the cytoprotective effects of taurine on hepatocytes during oxygenation, hypoxia, and on these paradoxes. The calcium and oxygen paradoxes were clearly revealed in isolated rat hepatocytes. As the formation of the superoxide radical was accelerated by the hyperbaric conditions and the calcium ions, drugs such as superoxide dismutase and a calcium channel blocking agent (verapamil), prevented these paradoxes. Taurine decreased the oxygenation-induced lipid peroxidation of hepatocytes, and prevented the hypoxia-induced hepatocyte death in a calcium-containing medium, but not in a calcium-free medium. Taurine also protected the hepatocytes from injuries associated with the calcium and oxygen paradoxes due to the inhibition of sudden calcium influx into the hepatocytes.

摘要

我们的研究旨在确定分离的大鼠肝细胞中的钙悖论和氧悖论，并评估牛磺酸在氧合、缺氧期间对肝细胞以及对这些悖论的细胞保护作用。在分离的大鼠肝细胞中清楚地揭示了钙悖论和氧悖论。由于高压条件和钙离子加速了超氧阴离子自由基的形成，超氧化物歧化酶和钙通道阻滞剂（维拉帕米）等药物可预防这些悖论。牛磺酸可降低氧合诱导的肝细胞脂质过氧化，并在含钙培养基中预防缺氧诱导的肝细胞死亡，但在无钙培养基中则不能。由于抑制了钙离子突然流入肝细胞，牛磺酸还可保护肝细胞免受与钙悖论和氧悖论相关的损伤。